^(60)Co-γ射线-离子束复合诱变法选育高产糖化酶菌株的研究  

Study on breeding of high-yield Gucoamlase strains by ^(60)Co-γ-ray and ion beam complex mutagenesis

作　　者：赵培城[1] 夏娟[1] 邓颜威[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310014

出　　处：《粮食与食品工业》2013年第4期82-85,共4页Cereal & Food Industry

摘　　要：以黑曲霉UV-4为出发菌株,利用60Co-γ射线与离子束进行复合诱变。试验表明:经复合诱变,可以获得高产糖化酶的菌株AnUVγN+7,酶活达到1 100.9U/mL,是原始菌株UV-4(156.1U/mL)的7倍,菌株经过7次传代,结果显示酶活较稳定,以此可选育高产糖化酶菌株。A mutant strain aspergillus niger with high yide of raw starch-digesting glucoamylase was isolated from protoplast of aspergillus niger UV-4 treated by ^60Co-γ-ray and ion beam. The raw starch-digesting glucoamylase activity of the strain raised to 1 100.9 U/mL, which was 7 times higher than the enzyme activity of protolast strin （156.1 U/mL）. The enzyme activity of the mutant strain was steady cultured by 7 times.

关 键 词：糖化酶 60Co-γ射线 离子束诱变 

分 类 号：TS20[轻工技术与工程—食品科学] TQ920[轻工技术与工程—食品科学与工程]