正交试验优化酵母多糖锌配合物的制备及其对尿素的吸附性能  

Preparation of Yeast Polysaccharide-Zn Complex and Its Adsorption Performance for Urea

作　　者：李兴艳[1] 张丙云[2] 尚永彪[1,3,4] 

机构地区：[1]西南大学食品科学学院,重庆400716 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050 [3]农业部农产品贮藏保鲜质量安全评估实验室(重庆),重庆400716 [4]重庆市特色食品工程技术研究中心,重庆400716

出　　处：《食品科学》2013年第14期57-62,共6页Food Science

基　　金：农业部兔产业技术体系岗位科学家项目(CARS-44-D-1)

摘　　要：为酵母多糖应用拓宽范围,以酵母多糖和硫酸锌为原料制备酵母多糖锌配合物,探讨了反应时间、锌的添加量、反应温度、pH值4个因素对配合物制备的影响。通过正交试验,确定制备锌配合物的最优条件。以尿素为吸附对象,研究酵母多糖锌配合物的吸附性能,考察了时间、温度、pH值、尿素质量浓度4个因素对配合物吸附尿素的影响,通过正交试验,确定酵母多糖锌配合物吸附尿素的最优条件。结果表明:酵母多糖锌配合物制备的最佳条件为温度55℃、反应时间2h、0.1mol/L硫酸锌的添加量10%、pH6.5,在该条件下所得到的酵母多糖和锌离子结合率为98.97%。酵母多糖锌配合物对尿素的最佳吸附条件为:温度30℃、pH6.0、时间135min、尿素的初始质量浓度2.25mg/mL,在该条件下对尿素的吸附率为97.27%。此时酵母多糖和锌离子的结合率较高,且该配合物对尿素具有很好的吸附作用。Yeast cell wall polysaccharide contains some groups that can combine metal ions for executing bioactive functions such as immunity.Yeast polysaccharide-Zn complex was prepared in this study to explore the absorption performance of urea and to extend the application of yeast polysaccharide.Yeast polysaccharide-Zn complex is composed of yeast polysaccharide and zinc sulfate.The influential factors of complex preparation such as reaction time,zinc addition amount,reaction temperature and reaction pH were investigated.The optimal conditions of zinc complex preparation were explored by orthogonal tests using urea adsorption as the evaluation index.The results indicated that the optimal preparation conditions of yeast polysaccharide-Zn complex required reaction temperature of 55 ℃,reaction time of 2 h,addition of 10% of 0.1 mol/L zinc sulfate,and reaction pH of 6.5.Under the optimal reaction conditions,the binding rate was up to 98.97%.The optimal adsorption conditions of yeast polysaccharide-Zn complex for urea were reaction temperature of 30 ℃,reaction pH of 6.0,reaction time of 135 min and initial urea concentration of 2.25 mg/mL.Under these optimal conditions,the adsorption rate was up to 97.27%.The higher binding rate between yeast polysaccharide and zinc ion can provide the better adsorption for urea.

关 键 词：酵母多糖 锌离子 酵母多糖锌配合物 制备 尿素 吸附 

分 类 号：TS201.2[轻工技术与工程—食品科学]