正交试验优化葛根素的大孔树脂纯化工艺  

Purification of Puerarin by Macroporous Resins

作　　者：李适[1,2] 岳明珠[1] 李银花 傅冬和[1] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]国家植物功能成分利用工程技术研究中心,湖南长沙410128

出　　处：《食品科学》2013年第16期89-92,共4页Food Science

基　　金：湖南省教育厅科研项目(11C0668);"十二五"国家科技支撑计划项目(2011BAD10B00)

摘　　要：以葛根粗提物为原料,比较D101、X-5、HD-101、D14、S-8、HC-D301、HD-8、D141、HPD100、HPD500、AB-8及NKA-2共12种大孔吸附树脂对葛根素的吸附与解吸性能。通过单因素试验、正交试验与验证实验,优化树脂分离纯化葛根素的工艺技术参数。结果表明:NKA-2树脂吸附效果最好,静态饱和吸附量可达184.9mg/g,在乙醇体积分数50%时的解吸率达90.86%;采用NKA-2树脂分离纯化葛根素的的最优参数为:上样液质量浓度18mg/mL,乙醇体积分数60%,洗脱速率1BV/h,此时葛根素的得率可达90.06%,纯度可达82.18%。该工艺具有较好的实用性与参考价值。Macrosporous resin types D101,X-5,HD-101,D14,S-8,HC-D301,HD-8,D141,HPD100,HPD500,AB-8 and NKA-2 were compared for their adsorption and desorption capacities for the purification of puerarin from a crude extract of RadixPuerariae.NKA-2 was found to have the strongest ability to adsorb puerarin with a static saturated adsorption quantity of 184.9 mg/g and a desorption percentage of 90.86% by 50% ethanol.The optimal conditions for purifying puerarin with NKA-2,as determined by orthogonal array design,were 18 mg/mL,60% ethanol and 1 BV/h for sample concentration,desorbent and desorption flow rate,respectively.Under the optimized conditions,the recovery and purify of puerarin were 90.06% and 82.18%.The optimized process has potential practical applications for the purification of puerarin.

关 键 词：大孔吸附树脂 葛根素 纯化 工艺参数 

分 类 号：TS202.3[轻工技术与工程—食品科学]