香蕉皮黄酮的分离纯化及抑菌活性研究  

Purification and Antimicrobial Activity of Total Flavonoid from Banana Peel

作　　者：顾采琴[1] 谢琳琳[1] 张织芬[1] 朱冬雪[1] 刘鹏[1] 赖雅平[1] 曾庆祝[1] 

机构地区：[1]广州大学化学化工学院食品系,广东广州510006

出　　处：《食品科学》2013年第16期98-102,共5页Food Science

基　　金：广州市科技计划资助项目(7411789649763)

摘　　要：以香蕉皮为原料,通过比较X-5、AB-8、H103、D101大孔树脂对香蕉皮黄酮静态吸附率和解吸率的影响,筛选出适宜分离纯化香蕉皮黄酮的大孔树脂,并通过单因素和多因素正交试验优化分离纯化工艺参数,同时测定了香蕉皮黄酮纯化前后对3种常见微生物的抑菌活性。结果表明:H103树脂为分离纯化香蕉皮黄酮的最佳树脂,其对香蕉皮黄酮的最大吸附量为3.66mg/g,平衡吸附时间约3h,解吸率达89.11%,解吸平衡时间约2.5h;H103分离纯化香蕉皮黄酮的动态吸附和解吸最佳工艺参数为上样液pH7.0、上样流速2.0mL/min、洗脱流速3.0mL/min、洗脱剂乙醇体积分数75%,此条件下黄酮回收率为72.50%,所得黄酮纯度为73%。香蕉皮黄酮对酵母、枯草芽孢杆菌和大肠杆菌均有一定的抑制作用,黄酮纯化后的抑菌效果优于纯化前。The best macroporous resin for purification of banana peel flavonoid（BPF） was selected from X-5,AB-8,H103 and D-101 by static adsorption and desorption capacities.H103 was identified as the best resin for BPF purification.The adsorption equilibrium was reached in about 3 h and the maximum adsorption quantity for BPF was 3.66 mg/g,while the time required to reach desorption equilibrium was about 2.5 h,yielding a desorption percentage of 89.11%.The optimal conditions for purifying BPF by dynamic adsorption and desorption on H103 were determined as 7.0,2.0 mL/min,3.0 mL/min and 75% ethanol for pH sample loading flow rate,desorption flow rate and desorbent,respectively.Under these conditions,the recovery and purity of BPF were 72.50% and 73%,respectively.BPF could inhibit the growth of Candidaalbicans,Escherichiacoli,Bacillussubtilis,and purified BPF had better antimicrobial activity.

关 键 词：香蕉皮黄酮 大孔树脂 分离纯化 抑菌活性 

分 类 号：O623.54[理学—有机化学]