胶体金免疫层析法定量检测孔雀石绿  

Development of Colloidal Gold Lateral Flow Assay for Quantitative Detection of Malachite Green

作　　者：山珊[1] 彭涛[1] 杨万春 刘春梅 熊勇华[1] 赖卫华[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]江西中德生物工程有限公司,江西南昌330029

出　　处：《食品科学》2013年第16期160-163,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAK10B01-03)

摘　　要：基于竞争抑制反应原理,制备孔雀石绿胶体金免疫层析试纸条,通过胶体金读取仪,读取试纸条上检测线(T线)及质控线(C线)的吸光度,建立孔雀石绿免疫层析定量检测方法。使用胶体金读取仪在多个时间段对不同质量浓度的孔雀石绿标品进行检测。以标品的不同质量浓度为横坐标,分别以T线吸光度、T/C值和T/(T+C)值为纵坐标建立3种检测模式。结果表明:检测时间为10min时,T线吸光度与标品质量浓度关系曲线的R2=0.9438,变异系数为1.6%～8.2%;T/C值与标准品质量浓度关系曲线的R2=0.9811,变异系数为0.4%～3.7%;T/(T+C)值与标准品质量浓度关系曲线的R2=0.9916,变异系数为0.3%～2.2%,和其前两种模型比较,最后一种方法的线性关系好,变异系数小,检测灵敏度为3μg/L,是一种较为理想的检测模型。Based on competitive binding immunoassay, a colloidal gold lateral flow strip for the quantitative detection of malachite green was developed by reading the absorbance of test （T） and control （C） lines with a test strip reader. Different concentrations of malachite green standard were detected with the test strip reader at different times of reaction. Three different detection models were proposed by plotting the absorbance of T line, T/C and T/（T ＋C） against the concentration of standard, respectively. At 10 min of reaction, the linear correlation coefficient （R^2） between the concentration of standard and the absorbance of T line was 0.9438 with coefficient of variation （CV, n = 3） of 1.6%-8.2%, whereas the r values for T/C and T/ （T ＋ C） were 0.9811 and 0.9916 and the CV values were 0.4%-3.7% and 0.3%-2.2%, respectively. The T/（T ＋ C） model exhibited better linearity and smaller CV, and its sensitivity was 3 μg/L.

关 键 词：孔雀石绿 免疫层析法 定量检测方法 

分 类 号：TS207.5[轻工技术与工程—食品科学]