改进的QuEChERS-气相色谱法测定果蔬保鲜剂抑霉唑残留  

Determination of Imazalil Residue in Fruits and Vegetables by Gas Chromatography with Modified QuEChERS

作　　者：徐小艳[1] 陈巧真[1] 梁晓瑜[1] 杨敏 

机构地区：[1]华南农业大学食品质量与安全研究所,广东省食品质量安全重点实验室,广东广州510640 [2]广州市农业科学研究院,广东广州510308

出　　处：《食品科学》2013年第16期247-250,共4页Food Science

基　　金：广东省农业标准化项目(F11037)

摘　　要：建立果蔬保鲜剂抑霉唑残留的改进QuEChERS快速提取净化方法及气相色谱检测方法。样品用含1%乙酸的乙腈溶液提取,氯化钠和无水硫酸镁盐析后,取上清液,加入N-丙基乙二胺(PSA)与十八烷基硅胶键合相(C18)吸附剂净化,用配有电子捕获检测器的气相色谱测定,外标法定量。抑霉唑在0.2～5.0mg/L(r=0.9994)范围内与峰面积呈良好线性关系;方法检出限(RSN≥3)为0.02mg/kg;回收率为74.3%～86.7%;相对标准偏差小于10%。该方法操作简便、准确,并且成本较低,适用于果蔬保鲜剂抑霉唑残留的测定。A rapid analytical method based on modified quick easy cheap effective rugged and safe（QuEChERS） sample preparation and gas chromatography（GC） for the analysis of imazalil residue in fruits and vegetables has been developed.Samples were extracted with acetonitrile containing 1% acetic acid and salted out by anhydrous MgSO4 and NaCl.Then,after clean-up on N-propyl ethylenediamine（PSA） cartridge and octadecyl silica bonded phase（C18）,the imazalil residue in the supernatant was subjected to GC-ECD analysis.An excellent linearity was achieved for imazalil in the range of 0.2–5.0 mg/L（r = 0.9994）.The detection limit was 0.02 mg/kg.The average spike recovery rates were in the range of 74.3%–86.7% with relative standard deviation less than 10%.The proposed method was simple,accurate,low-cost and applicable for the determination of imazalil residue in fruits and vegetables.

关 键 词：抑霉唑 QUECHERS 气相色谱 果蔬 保鲜剂 

分 类 号：O657.72[理学—分析化学]