鼻咽癌染色体3p14的精细等位基因缺失研究  

Precise map of allelic loss on chromosome 3p14 in nasopharyngeal carcinoma

作　　者：邓燕飞[1] 田芳[2] 杨新明[1] 谢鼎华[1] 卢永德[1] 邵细芸[2] 陈主初[2] 

机构地区：[1]湖南医科大学附属第二医院耳鼻咽喉科,长沙410011 [2]湖南医科大学肿瘤研究所细胞生物学研究室

出　　处：《中华耳鼻咽喉科杂志》2000年第5期391-393,共3页Chinese Journal of Otorhinolaryngology

基　　金：国家自然科学基金!(39500172);卫生部课题基金!(961131)

摘　　要：目的　研究鼻咽癌染色体 3p14区域的精细等位基因杂合性丢失 (lossofheterozygosity,LOH)情况 ,并探讨LOH与鼻咽癌临床分期、临床病理和EB病毒 (Epstein Barrvirus,EBV)感染的关系。方法　采用 3p14区域 6个精确高密度的微卫星多态性位点 ,对 32例患者鼻咽癌组织进行LOH分析。结果　 32例患者中有 2 3例 ( 71 9% )在至少 1个位点发生LOH ,丢失频率较高的 3个位点是D3S1313( 46 4% )、D3S130 0 ( 5 0 0 % )和D3S1312 ( 44 4% )。在具有丢失的 2 3例患者中 ,12例表现为一个连续的非随机的LOH区域 ,其最小共同缺失区为D3S1313～D3S1312。该区域的LOH与临床分期、EBV感染有明显关系。 30例低分化鳞癌的LOH频率为 70 0 % ,2例泡状核细胞癌均存在 2个位点的LOH。结论　鼻咽癌染色体 3p14区存在较高的LOH率 ,提示在D3S1313和D3S1312之间可能存在尚未克隆的与鼻咽癌发生发展相关的抑癌基因。To determine the precise allelic loss on chromosome 3p14 and discuss the possible relations between loss of heterozygosity (LOH) and EBV infection, clinical stage and clinic-pathology of nasopharyngeal carcinoma (NPC) Methods Six high dense microsatellite marker on chromosome 3p14 were selected to examine LOH in 32 cases of NPC Results 23 of 32 (719%) tumors were deleted for at least one locus of six loci High frequencies of LOH(>40%) were observed at loci D3S1300(500%),D3S1313(464%) and D3S1312(444%)12 cases showed LOH in one contiguous and nonrandom region. The smallest common deletion region seems likely to lie between D3S1313 and D3S1312 Relations between LOH on 3p14 and clinical stage and EBV infection were observed The frequency of LOH was 700% in 30 cases of poor differentiated squamous cell carcinoma 2 cases of vesicular nucleus cell carcinoma had LOH at two loci Conclusion The high deletion rate on 3p14 in NPC indicates that there might be a putative tumor suppressor gene related to the development and progression of NPC

关 键 词：鼻咽肿瘤 染色体 杂合性丢失 等位基因缺失 

分 类 号：R739.6[医药卫生—肿瘤]