酵母菌中SOD复合酶的初步研究  

A preliminary study on the compound enzyme of superoxide dismutase from yeast

作　　者：王岁楼[1] 张平之[1] 张鑫[1] 何克进 

机构地区：[1]郑州轻工业学院食品与生物工程系,郑州450002

出　　处：《生物学杂志》2000年第4期26-27,共2页Journal of Biology

基　　金：河南省自然科学基金项目(豫科 9840 3330 0号)

摘　　要：对不同酵母菌中SOD等抗氧化酶的活性进行了初步的分析测定 ,筛选出了一株诸酶活性都较高的菌株 (丹宝利面包活性干酵母 )。研究了该酵母在不同培养时期SOD等酶活力的变化情况 ,发现POD、CAT等酶的活性水平与SOD活性的变化有密切的相关性。通过比较几种提取方法的效果 ,认为利用甲苯破壁法提取SOD复合酶具有一定的可行性。The relation of superoxide dismutase(SOD) with peroxidase (POD) and catalase(CAT) is simply studied by active dry yeast.Under the different period of culture time,the activities of POD and CAT have close relation with that of SOD.During log-growth phase,the activity of SOD increases with culture time,and at the same time the activities of the other two enzymes also increase.After the 1og-growth phase,the SOD activity decreases slightly,and the other two enzymes have similar characteristics.We call the enzyme preparation containing SOD,POD and CAT etc.As the compound enzyme of SOD,and therefore crude extract of SOD from yeast belonge to the compound enzyme of SOD.

关 键 词：酵母菌 超氧化物歧化酶 过氧化物酶 SOD5复合酶 

分 类 号：Q554[生物学—生物化学] Q936