葡萄子浸提液对亚硝化反应的抑制作用  

Study on Inhibitory Effect of Nitrosation Reaction by Extracts of Grape (Vitis vinifera) Seeds

作　　者：张成[1] 徐子梁[1] 温广辉[1] 杨虹[1] 许华[1] 张梦诗[2] 颜亨梅[1] 

机构地区：[1]北京师范大学珠海分校工程技术学院生物工程系,广东珠海519087 [2]广东省江门中医药学校,广东江门529000

出　　处：《湖北农业科学》2013年第14期3381-3385,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31172107);北京师范大学珠海分校青年教师科研项目(201353015);北京师范大学珠海分校重点基金项目(Z07002)

摘　　要：采用L16(45)正交试验浸提葡萄(Vitis vinifera)子中的活性成分,并测定了葡萄子浸提液对亚硝酸钠的清除率以及对N-亚硝基化合物合成的阻断率,进而通过极差分析确定最佳浸提工艺条件。以对亚硝酸钠的清除率为考察指标,葡萄子活性成分的最佳浸提工艺条件为料液比1∶25(m/V,g∶mL),在沸腾回流条件下浸提2.0 h,该活性浸提液对亚硝酸钠的最大清除率为90.2%。以对N-亚硝基化合物合成的阻断率为考察指标,葡萄子活性成分的最佳浸提工艺条件为料液比1∶25(m/V,g∶mL),在60℃下浸提2.0 h,该活性浸提液对N-亚硝基化合物合成的最大阻断率为86.0%。The optimum conditions for extracting active components of grape （Vitis vinifera） seeds were determined by L16（d4） orthogonal test. The scavenging effect to sodium nitrite and inhibitory effect to N-nitroso compounds formation of the extracts of grape seeds were investigated. The results showed that when considering the scavenging rate as the main index, the opti- mum extraction conditions of effective compounds from grape seeds were, the ratio of solid to solution at 1：25 （m/V,g：mL）, and extracting by boiling reflux for 2 h. Under these conditions, the maximum scavenging capacity to sodium nitrite was 0.902. When considering the disconnection rate to N-nitroso compounds formation as the main index, the optimum extraction conditions were, the ratio of solid to solution at 1：25 （m/V,g：mL）, and extracting at 60 ℃ for 2 h. Under these conditions, the maximum inhibitory capacity to the formation of N-nitroso compounds was 0.860.

关 键 词：葡萄(Vitis vinifera)子浸提液 阻断 清除 亚硝酸钠 N-亚硝基化合物 

分 类 号：O623.54[理学—有机化学] S663.1[理学—化学]