高效液相色谱法同时测定果蔬与血浆中的番茄红素和β-胡萝卜素  

The simultaneous determination of lycopene and β carotene in fruit vegetables and plasma by HPLC.

作　　者：李向荣[1] 方晓[1] 陈青俊[2] 黄百芬 

机构地区：[1]浙江大学医学院,浙江杭州310031 [2]浙江省轻工业研究所,浙江杭州310011

出　　处：《浙江大学学报（农业与生命科学版）》2000年第4期444-446,共3页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省科委资助项目!(980 5 5 );浙江省教委资助项目!(981 0 6 5)

摘　　要：为了建立一种快速分离和分析果蔬食物与血浆中番茄红素和 β 胡萝卜素的方法,对不同样品采用不同的前处理:以ODS C18为色谱柱,乙腈∶四氢呋喃(60∶4 0,V/V)为流动相,在 1.0mL/min的流速下,选择 4 45nm检测。番茄红素和 β 胡萝卜素的回收率分别为 89.54%～ 93.71 %和 90.2 9%～ 96.4 3 %,变异系数为 3.9%～ 5.68%。番茄红素和 β 胡萝卜素能很好地分离。高效液相色谱法简单准确,适合于果蔬食物与血浆中番茄红素和 βTo establish a rapid determination method of lycopene and β carotene in fruit vegetables and plasma, different pretreatments were used for different samples. Separation was achieved by the use of ODS C 18 column, acetonitrile: tetrathydrofuran (60∶40, V/V) as mobile phase, a flow rate of 1.0 mL/min and detector at 445 nm was used. The recoveries of lycopene and β carotene were 89.54% 93.71% and 90.29% 96.43% respectively, the coefficients of variation were 3.9% 5.68%. The results showed that this method was simple, rapid and accurate.

关 键 词：高效液相色谱法 番茄红素 Β-胡萝卜素 番茄 

分 类 号：Q562.03[生物学—生物化学] R151.3[医药卫生—营养与食品卫生学]