产β-1,3-1,4-葡聚糖酶特基拉芽孢杆菌Bacillus tequilensis CGX5-1发酵培养基的优化  

Medium Optimization for the Production of β-1,3-1,4-Glucanase by Bacillus tequilensis CGX5-1

作　　者：刘晓玲[1,2] 王金晶[1,2] 李永仙[1,2] 李崎[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《食品与生物技术学报》2013年第6期645-650,共6页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2012AA021303);教育部新世纪人才支持计划项目(NCET-10-0453);国家创新基金项目(09C26213203751);江苏省创新基金项目(BC2009291);江苏高校优势学科建设工程资助项目(PAPD);江南大学自主科研计划项目(JUSRP11218)

摘　　要：采用响应面优化法对一株野生特基拉芽孢杆菌的发酵培养基进行优化,最终培养基各组分为:大麦粉68.4 g/L,玉米粉40 g/L,豆饼粉61.1 g/L,KH2PO41 g/L,MgSO4·7H2O 0.1 g/L,CaCl20.1 g/L。用优化培养基在37℃摇瓶发酵52 h,β-1,3-1,4-葡聚糖酶酶活达到191.96 U/mL,是优化前产酶水平的1.91倍。Bacillus tequilensis CGX5-1 is a newly screened strain which can effectively secrete β-1,3-1,4-glucanase. Fractional factorial design was used to clarify the medium components and the concentration of harley flour and soy bean flour were found to be the key factors. The steepest ascent experiments was applied to determine the optimal domain and the central composite design was used to estimate the quadratic response surface, thus we acquire the factor values for maximum production of β-1,3-1,4-glucanase. The final composition of fermentation medium was determined via response surface methodology,which was （g/L） ：harley flour 68.4,corn flour 40,soybean flour 61.1,KHzPO4 1 ,MgSO4·7H2O 0.1 ,CaCl2 0.1. The highest activity of β-1,3-1,4-glucanase was 191.96 U/mL at 52 h culture using optimized medium,which was 1.91 times higher than that from original medium.

关 键 词：Β-1 3-1 4-葡聚糖酶 响应面法 培养基优化 特基拉芽孢杆菌 

分 类 号：TS201.25[轻工技术与工程—食品科学]