发酵过程流加L-谷氨酸提高ε-聚赖氨酸的产量  

Enhancement of ε-poly-L-lysine production through L-glutamic acid feeding during fermentation by Streptomyces sp. M-Z18

作　　者：董难[1] 陈旭升[1] 任喜东[1] 曾昕[1] 孙启星[1] 张建华[1] 毛忠贵[1] 

机构地区：[1]江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与发酵工业》2013年第7期79-82,共4页Food and Fermentation Industries

基　　金：江苏省科技支撑计划(BE2012616);无锡市科技支撑计划(CYE21N1107);江南大学自主科研计划(JUSRP11217)资助

摘　　要：为了提高Streptomyces sp.M-Z18合成ε-聚赖氨酸(ε-PL)能力,在考察L-谷氨酸添加浓度和添加时机基础上,提出发酵过程中流加L-谷氨酸的策略。结合甘油补料-分批发酵方式,该策略实现174 h内ε-PL发酵产量和产率分别达到31.65 g/L和4.36 g/(L·d),较原发酵工艺分别提高49.2%和43.9%。实验结果表明,发酵过程流加L-谷氨酸是提高ε-PL发酵水平的有效策略之一。To improve the production of ε-poly-L-lysine （ε-PL） by Streptomyces sp. M-Z18, the strategy of L- glutamic acid feeding during fermentation was developed with the help of optimization of L-glutamic acid supplement concentration and time. Combined with fed-batch fermentation using glycerol as carbon source, the ε-PL production and productivity was reached to 31.65 g/L and 4.36 g/（L . d） respectively in 174 h. Compared with the fermentation without L-glutamic acid addition, the data were increased by 49.2% and 43.9% , respectively. These resuhs demonstrated that the feeding of L-glutamic acid during fermentation was a novel approach for improve ε-PL fermentation level by ε-PL producing strains.

关 键 词：ε-聚赖氨酸 L-谷氨酸流加 STREPTOMYCES SP M-Z18 甘油 补料-分批发酵 

分 类 号：TQ922[轻工技术与工程—发酵工程]