鲜活农产品中大肠杆菌的PCR检测  

PCR detection of E.coli in fresh products

作　　者：郝江燕[1,2] 胡文忠[2] 何煜波[2] 冯叙桥[1] 忻文静[2] 

机构地区：[1]沈阳农业大学食品科学学院,辽宁沈阳110866 [2]大连民族学院生命科学学院,辽宁大连116600

出　　处：《食品工业科技》2013年第17期150-153,共4页Science and Technology of Food Industry

基　　金：国家科技支撑计划项目(2012BAD38B05);国家自然科学基金项目(31172009);大连市科技计划项目(2012E13SF106)

摘　　要：实验利用常规PCR进行大肠杆菌检测。确定了此检测方法的引物和检测限,改进了DNA模板的提取方法,并将其应用在采自大连市开发区农副产品批发市场、金发地批发市场、金马商城农贸市场、乐购超市以及新玛特超市的三类鲜活产品的检测。结果表明:以大肠杆菌特异性基因UidA为扩增片段,用PCR检测方法检测大肠杆菌的检测限为6.8×102CFU/mL;三类鲜活产品在6h增菌的处理下检测发现,畜禽类产品中大肠杆菌阳性率为75%,水产类产品中大肠杆菌阳性率约为56.2%,果蔬中大肠杆菌阳性率约为47.8%。PCR detection method was used to detect E.coil in series of fresh products.The primer and detection limit were determined,in the meantime,the DNA template extraction method was improved and these was applied to products purchased from Da Lian Development Zone Agricultural and Sideline Products Wholesale Market, Jin Fadi Wholesale Market, Golden Horse Mall Farmer＇ s Market, Tesco Supermarket and New Matt Supermarket. The results showed that：the assay was performed with a E.cofi-specific UidA gene amplicon, detection limit for this assay was 6.8×10^2CFU/mL.Three series of fresh products were detected using this method after 6h enrichment, the results showed that 75% of positive in livestock and poultry,56.2% of positive in aquatic products and 47.8% of positive in fruits and vegetables.

关 键 词：鲜活农产品 大肠杆菌 PCR 

分 类 号：TS207.4[轻工技术与工程—食品科学]