高效酒精酵母菌选育及其特性研究  

Screening of efficient alcohol-producing yeast and its characteristics

作　　者：罗跃中[1] 李忠英[1] 李继睿[1] 吴永尧[2] 

机构地区：[1]湖南化工职业技术学院,湖南株洲412004 [2]湖南农业大学生命科学院,湖南长沙410128

出　　处：《中国酿造》2013年第8期71-74,共4页China Brewing

基　　金：湖南化工职业技术学院院级课题项目(Hnhgzy200904)

摘　　要：从来源不同的基质中采用耐热、耐酒精处理方法，结合氯化三苯基四氮唑（TTC）法筛选得到1株酒精发酵的高产酵母菌株TJ-18。对该菌株进行紫外线和亚硝酸复合诱变处理，得到1株稳定高产突变株UV-N-5，其产酒精度达8．25％vol，比出发菌株提高39．83％；同时对其生理特性进行研究发现，该菌可在pH值为3．0-9．0的范围内正常生长，在糖浓度达到40％时，温度40℃时仍保持出很好的耐受性。且在12％vol和8％vol的酒精浓度时，UV-N-5的死亡率分别是34％和11％，对酒精耐受性突出。这些特点都符合现代酒精发酵酵母的要求。Strain TJ-18 was screened by the methods of triphenyltetrazolium chloride （TTC）, using heating and alcohol resistances from the different source of matrix. By the ultraviolet ray and nitrous acid compounding mutation, a stable high alcohol yield mutant strain UV-N-5 was gained which the alcohol content reached to 8.25%vol, 39.83% higher than starting strain. The research of physiological characteristics showed that the yeast could growth normally in pH value 3-9, and kept a good resistance under the 40%-45% sugar concentration at 40℃. UV-N-5 had a resistance to alcohol of which the death rate was respectively 43% and 11% under the 12% and 8% of the alcohol concentration. All these characteristics could meet the re- quirement of yeast for modem alcoholic fermentation.

关 键 词：耐高温酵母 筛选 紫外线 亚硝酸 复合诱变 酒精发酵 

分 类 号：Q93-331[生物学—微生物学]