乳化法制备交联海藻糖合酶聚集体的研究  

Cross-linked Trehalose Synthase Aggregates Prepared by Emulsification

作　　者：陈晓云[1] 陈颖[1] 肖辰鹏[2] 杨丽维[1] 李明春[2] 唐柳[1] 张峻[1] 

机构地区：[1]天津市林业果树研究所,天津300384 [2]南开大学微生物学系,天津300071

出　　处：《南开大学学报（自然科学版）》2013年第2期107-112,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市应用基础及前沿技术研究计划(10JCYBJC09600;10JCYBJC05000);国家自然科学基金(27076162)

摘　　要：交联酶聚集体（CLEAs）是一种高活性的无载体固定化酶，常用的基于溶液法（Sheldon法）制备的CLEAs呈无定形的集簇体，影响其连续使用效率．以海藻糖合酶为模型酶，首次在油包水乳化体系中构建了CLEAs，利用扫描电镜和光学显微镜分析了所得CLEA的微观结构，考察了CLEAs的活性和重复使用性能．结果表明，乳化法制备的CLEAs外观呈较规则的微球形，粒径约20～60μm，其活性和重复使用性能均高于参照Sheldon法制备的CLEAs．Cross-linked enzyme aggregates （CLEAs） is a kind of carrier-free immobilized enzyme with high activity. However,the CLEAs prepared by solution based process （Sheldon＇s method） forms into amorphous clusters,affecting its efficiency of continuous utilization. A new approach to construct CLEAs in water-in-oil emulsion was proposed, and the microstructure and catalytic properties of CLEAs of trehalose synthase prepared by the above two methods were investigated. Optical and scanning electron microscopy of CLEAs revealed that the CLEAs obtained by emulsion based process formed into structured spherical particles between 20~ 60μm in diameter. Its activity and reusability were higher than that of the CLEAs prepared us- ing Sheldon＇s method.

关 键 词：海藻糖合酶 交联酶聚集体 油包水乳液 

分 类 号：Q814.2[生物学—生物工程]