一株沙门氏菌烈性噬菌体的分离纯化与生理特性研究  

Isolation,Identification and Physiological Characterization of Lytic Phage against Bacterium Salmonella sp.

作　　者：李萌[1] 韩峰[1] 林洪[1] 王静雪[1] 

机构地区：[1]中国海洋大学食品科学与工程学院食品安全实验室,山东青岛266003

出　　处：《水产科学》2013年第9期531-535,共5页Fisheries Science

基　　金：国家自然科学基金资助项目(31071540);教育部博士点专项基金资助项目(No.20100132110013);现代农业产业技术体系建设专项资金资助项目(No.nycytx-50)

摘　　要：自水产品市场的污水中采用双层平板法分离纯化出一株烈性沙门氏菌噬菌体SP3。利用电镜观察其形态特征，SDS-PAGE电泳和琼脂糖凝胶电泳分析其结构蛋白和核酸成分，并测定其生理特性。包括一步生长曲线、热和pH稳定性、抑菌活性和最适保存条件。结果显示该烈性噬菌体SP3为双链DNA，属于长尾噬菌体科，结构蛋白为43．0～66．2ku。该株噬菌体潜伏期5rain，裂解期为75min，最适培养温度和pH分别为30-40℃和7～9，在液体环境中能够高效抑制沙门氏菌的生长。不同的保存方法显示，SP3在-20℃低温保存条件下活性保持较好。A lytic phage SP3 was isolated from effluent from a fishery product market by a double-layer plate method to develop an effective controlling method against bacterium Salmonella sp. The SP3 were morphologically observed by transmission electron microscope, and the composition of protein and nucleic acid were analyzed by SDS-PAGE and AGE. Furthermore some physiological properties were also characterized including one step curve, pH stability, thermal stability, inhibition activity and storage condition. The SP3 was found to be a member of family Siphoviridae, whose main proteins were ranged from 43. 0 to 66. 2 ku and the deoxyribose nucleic acid （DNA） was digested with the restriction endonuclease. The latent time period and burst time were calculated to be 5 and 75 min, respectively. The SP3 was stable at a wide range of temperatures from 30 to 40 ℃ and pH 7 - 9. At liquid culture conditions, the bacterium treated with phage had much lower optical density than the control one. The storage condition test revealed that the SP3 should be stored at --20 ℃.

关 键 词：沙门氏菌 烈性噬菌体 生理特性 

分 类 号：TS254.5[轻工技术与工程—水产品加工及贮藏工程]