65株冷冻肠衣分离细菌的鉴定  

Identification of 65 strains of bacteria isolated from frozen intestines

作　　者：仇保丰[1] 宋鸿雁[2] 蔡宝亮[3] 邵义祥[2] 顾炳泉[1] 刘文斌[1] 高逢结[1] 李建[1] 

机构地区：[1]南通出入境检验检疫局,江苏南通226004 [2]南通大学实验动物中心,江苏南通226001 [3]江苏出入境检验检疫局,江苏南京210001

出　　处：《中国食品卫生杂志》2013年第5期458-461,共4页Chinese Journal of Food Hygiene

基　　金：南通市科技项目(BK2012091);江苏检验检疫局科研项目(2010KJ35)

摘　　要：目的调查冷冻肠衣携带细菌的情况。方法用16S rDNA PCR序列扩增和分析技术对2010—2012年从南通地区肠衣生产、加工企业的冷冻肠衣中分离的65株细菌进行鉴定。结果这些细菌包含了来自13个属的20种细菌,其中奇异变形杆菌(Proteus mirabils)、海氏肠球菌(Enterococcus hirae)和粪肠球菌(Enterococcus faecalis)是比较明显的优势种群。另外,许多细菌在一定条件下可引起人和动物发生疾病。结论冷冻肠衣可以携带多种细菌,应该进一步加强监测和研究。Objective To study the bacteria contamination in frozen intestines.Methods Sixty-five strains of bacteria isolated from frozen intestines,which collected in Nantong casings manufacturing and processing enterprises between 2010 and 2012,were identified by 16S rDNA PCR amplification and sequence analysis.Results It showed that 20 specices of bacteria blonging to 13 genuses were identified,and the main bacterial species were Proteus mirabils,Enterococcus hirae and Enterococcus faecalis.In addition,some of them may cause disease in humans and animals under certain conditions.Conclusion A variety of bacteria may be carried by frozen intestines,the relevant supervision and study should be further strengthened.

关 键 词：冷冻肠衣 16SrDNA 食源性致病菌 

分 类 号：R155.55[医药卫生—营养与食品卫生学] TS251.92[医药卫生—公共卫生与预防医学]