高效液相色谱-电感耦合等离子体质谱分析大米中砷形态化合物  

Determination of arsenic species in rice by HPLC-ICP-MS

作　　者：侯艳霞[1,2] 刘丽萍[1] 潘浩[3] 杜振霞[2] 李筱薇[3] 

机构地区：[1]北京市疾病预防控制中心,北京100013 [2]北京化工大学理学院,北京100029 [3]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《分析试验室》2013年第10期103-107,共5页Chinese Journal of Analysis Laboratory

摘　　要：采用0.15 mol/L HNO3为提取剂,90℃热浸提大米样品2.5 h,Hamilton PRP-X100阴离子交换柱(250 mm×4.1 mm,10μm)分离,高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)分析检测大米中砷形态化合物。结果表明:大米中4种砷形态化合物(AsⅢ、AsⅤ、DMAⅤ、MMAⅤ)的检出限在0.06～0.08μg/L之间,加标回收率为73.7%～118.2%,RSD均小于6%,方法适用于大米中砷形态化合物的分析测定。It is a hot spot and difficulty to extract and analyze different species of arsenic rice compounds from both in domestic and loverseas.The separation of samples was performed on a Hamilton PRP-X100 column（250 mm x 4.1 mm,10 μm） with 0.15 mol / L nitric acid（HNO 3） as an extracting agent to pretreat rice samples at a temperature of 90 ℃ in oven for 2.5 h.High performance liquid chromatography（HPLC）-inductively coupled plasma mass spectrometry（ICP-MS） was used for analysis.The results showed that the detection limits of four main arsenic species（AsⅢ,AsⅤ,DMAⅤ,MMAⅤ） in rice were between 0.06 μg/kg and 0.08 μg/kg,the recoveries were in the range of 73.7% ～ 118.2%,and the relative standard deviation（RSD） was less than 6%.A simple,fast and accurate method for the analysis of arsenic species in rice was developed.

关 键 词：高效液相色谱-电感耦合等离子体质谱 大米 砷形态 

分 类 号：O657.63[理学—分析化学]