近红外光谱法定性定量检测蜂蜜中掺入甜菜糖浆的可行性研究  

Qualitative and Quantitative Detection of Beet Syrup Adulteration of Honey by Near-Infrared Spectroscopy:A Feasibility Study

作　　者：李水芳[1] 文瑞芝[1] 尹永 周孜 单杨[3] 

机构地区：[1]中南林业科技大学理学院,湖南长沙410004 [2]湖南明园蜂业有限公司,湖南长沙410025 [3]湖南省食品测试分析中心,湖南长沙410025

出　　处：《光谱学与光谱分析》2013年第10期2637-2641,共5页Spectroscopy and Spectral Analysis

基　　金：国家科技支撑计划项目(2009BADB9B07);中南林业科技大学人才启动基金项目资助

摘　　要：为进一步检验近红外光谱技术(NIRS)快速检测蜂蜜掺假的能力,利用近红外光谱结合化学计量学方法对蜂蜜中掺入甜菜糖浆进行了定性和定量检测。偏最小二乘-判别分析法(PLS-DA)对真假蜂蜜预测集的判别总正确率为90.2%;不同判别方法对掺假量等级预测集的判别总正确率都低于33.3%;PLS回归只对同一蜂蜜样本掺假的定量分析结果满意:预测集真实值与预测值的相关系数(r)为0.982 9,预测均方差(RMSEP)为1.394 2,而对不同植物来源和同一植物来源的不同样本的掺假量的定量分析结果不满意。研究表明,蜂蜜中掺入甜菜糖浆后,NIRS可实现真假蜂蜜的快速鉴别,而不能实现掺假量等级的鉴别及掺假量的定量分析。In order to further investigate the utility of near-infrared spectroscopys （NIRS） in rapidly detecting honey adultera- tion, near-infrared spectroscopy in combination with chemometric methods was investigated for qualitative and quantitative detec- tion of beet syrup adulteration of honey. Total prediction accuracy of testing set was 90. 2% by partial least squares-discriminant analysis （PLS-DA） for authentic and adulterated honey samples. Total prediction accuracy of testing sets was all below 33.3~ by different discriminant methods for classes of adulteration level. The quantitative analysis of adulteration level by PLS regres- sion gave satisfying results if adulterated honey samples were got from the same one authentic honey sample： correlation coeffi- cient （r）of actual values versus predicted values was 0. 9829 and root mean square error of prediction （RMSEP） was 1. 394 2 intesting set, otherwise it gave dissatisfying results for the adulterated samples from different botanical origins or the different samples of the same botanical origins. The results showed that NIRS could be applied for rapid detection of authentic and adul- terated honey samples, but not for detection of classes of adulteration level and quantification of adulteration level with beet syr- up.

关 键 词：近红外光谱 蜂蜜 掺假 甜菜糖浆 判别分析 定量分析 

分 类 号：O657.3[理学—分析化学]