转高赖氨酸融合蛋白基因水稻谷蛋白急性毒性  

Acute Toxicity of Glutelin in Transgenic Rice Expressing Lysine-rich Fusion Protein Gene

作　　者：赵祥祥[1] 陆长丽[1] 胡小兰[1] 纪丽莲[1] 刘巧泉[2] 唐瑭[1] 刘福霞[1] 

机构地区：[1]淮阴师范学院生命科学院江苏省环洪泽湖生态农业生物技术重点实验室,江苏淮安223300 [2]扬州大学农学院植物功能基因组学教育部重点实验室,江苏扬州225009

出　　处：《食品科学》2013年第17期284-288,共5页Food Science

基　　金：国家转基因生物新品种培育重大专项(2009ZX-08011-003B);江苏省高校"青蓝工程"中青年学术带头人培养对象资助项目

摘　　要：以我国自主培育的转高赖氨酸融合蛋白基因水稻(简称转GL基因水稻)为材料,参考中华人民共和国国家标准,通过小鼠急性经口毒性实验研究转GL基因水稻谷蛋白的食用安全性。采用低碱法(0.35%)提取的谷蛋白以5000mg/kg最大剂量灌胃小鼠,连续观察14d后测定小鼠体质量、血常规、脏器系数和脏器的病理学检查。结果表明:整个实验期间,小鼠生长状态良好,无死亡和异常现象;转GL基因稻米谷蛋白LD50>5000mg/kg,属实际无毒级别;各实验组间的小鼠体质量增长无明显差异,血常规指标、脏器系数和病理学检查也未见显著差异。因此,转GL基因水稻谷蛋白对小鼠无急性毒性作用。Lysine-rich rice is a transgenic rice produced by inserting lysine-rich fusion protein gene into the germline of rice seeds. According to the national standard of the People’s Republic of China, the food safety of glutelin extracted from this transgenic rice by low alkali method was evaluated using acute toxicity test. In this study, glutelin was administered to ICR mice by oral gavage at the dose of 5000 mg/kg. for continuous 14 d. Some indicators including body weight, routine blood test, relative organ weights and histopathological examinations were conducted. The results showed that no toxic symptom was found in tested mice in the acute toxicity test, and the LD50 for glutelin was higher than 5000 mg/kg. The glutelin from transgenic GL gene rice, therefore, could be classified as actual nontoxic. No adverse effect on animal behavior or mortality was observed during the experiment. There were no significant biological changes in body weight, routine blood parameters or relative organ weights and no histopathological damage was detected. Our results suggested that the glutelin from transgenic GL gene rice had no acute toxic effect on ICR mice.

关 键 词：转GL基因水稻 谷蛋白 急性毒性 食品安全 

分 类 号：TS201.6[轻工技术与工程—食品科学]