白腐真菌产木聚糖酶降解半纤维素及改善肠道微生态平衡作用  

Xylanase from Pleurotus ostreatus SYJ-042 Degrades Hemicellulose and Ameliorates Intestinal Microeological Balance

作　　者：李天歌[1] 岳晓禹[2,3] 李自刚[2] 蔡青和[3] 

机构地区：[1]四川农业大学食品学院,四川雅安625014 [2]河南牧业经济学院质检系,河南郑州450011 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2013年第17期313-316,共4页Food Science

基　　金：2011年河南省重点科技发展计划项目(112102110034);2011年度河南省教育厅自然科学研究计划项目(2011A550014;12A550007);河南牧业经济学院博士科研启动资金项目(5040190)

摘　　要：以白腐真菌菌株PleurotusostreatusSYJ-042为研究对象,对该菌株产木聚糖酶进行果皮和动物饲用效果研究。果皮效用实验结果表明:酶液对果皮粉中半纤维素的降解随反应时间的延长降解率增加,反应5h的降解率就达到了62.44%。动物实验结果表明:实验组大鼠的采食量比对照组要高,差异极显著(P<0.01),日增重比对照组要高,差异极显著(P<0.01);实验组大鼠对饲料中半纤维素降解率数值与对照组相比没有差异显著;与对照组相比,实验组大鼠空肠部位的总菌数有显著差异(P<0.01),盲肠部位的总菌数没有显著差异,结肠中总菌数要显著低于对照组(P<0.01)。因此,木聚糖酶有利于果皮的脱皮,能促进大鼠的生长,改善肠道微生态平衡。Xylanase has a promising potential of applications in the feed industry as well as the food and pulp industry. These applications of xylanase from Pleurotus ostreatus SYJ-042 were investigated. The results showed that the degradation of hemicellulose in pear pericarp was gradually increased with reaction time. The degradation rate at 5 h reached 62.44%. Animal experiments showed that the experimental group fed on xylanase-containing wheat diet had a higher food intake than the control group and the difference was significant. The daily weight gain was also higher than that of the control group and a significant difference was observed （P 〈 0.01）. However, the dietary hemicellulose degradation rate in the experimental group compared with the control group did not differ significantly. Administration of xylanase-containing diet also decreased the total bacterial count in the jejunum and colon （P 〈 0.01） without resulting in a significant difference for the cecum （P 〈 0.01）. In conclusion, the xylanase was beneficial to remove the pericarp, promote the growth of rats, and maintain intestinal microecological balance.

关 键 词：白腐真菌 木聚糖酶 应用 

分 类 号：Q814.9[生物学—生物工程]