响应面法优化巨大芽孢杆菌产纤维素酶发酵条件  

Optimization of Bacillus Megaterium Cellulase Production for Submerged Fermentation by Response Surface Methodology

作　　者：韩学易[1] 唐自钟[1] 胡云龙[1] 万玉军[1] 王琨[1] 陈虹[1] 陈惠[1] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《四川农业大学学报》2013年第3期319-321,334,共4页Journal of Sichuan Agricultural University

基　　金：四川省科技厅科技支撑项目(课题编号:2008GZ0150)

摘　　要：【目的】为了解决纤维素酶发酵工业产量低、生产成本高等问题，加快纤维素酶工业化应用的步伐。【方法】采用5L发酵罐，利用响应面分析法（RSM）对基因工程菌WH320—pHIS1525-G7从接种量、木糖流速及流加时问进行产酶优化。【结果】确定其发酵工艺为：17．62％的接种量，温度37℃，pH7．0，罐压0．03～O．05Mpa，转速300r／rain，0．41g／（L·h）的速度流加木糖11．33h，发酵过程中控制溶氧浓度≥30％。【结论】在该发酵条件下测得纤维素酶活力为2．152U／mL，比优化前摇瓶发酵酶活力提高了2倍，可为工业化生产纤维素酶提供依据。[Objective] To solve the low yield, high production cost issues in fermentation indus- try, and speed up the pace of industrial application of cellulase. [Method] The research used re- sponse surface methodology to optimize the fermentation conditions of neutral cellulase production by a Bacillus Megateriurn genetic engineering bacteria WH320-pHIS1525-G7 stain in 5 L fermen ter. [Results] The optimal fermentation conditions were 17.62% inoculation amount, 37℃ fer mentation temperature, pH 7.0, tank pressure 0.03--0.05 Mpa, rotation speed of shaker 300 r/ rain, fed 11.33 h xylose by 0.41 g/（L. h） flow rate, dissolved oxygen 30℃ during the fermen tation process. [Conclusion] The cellulase activity was up to 2. 152 U/mL which was 2 times higher than the shaking culture. This research can provide the basis for the cellulase manufacture.

关 键 词：巨大芽孢杆菌 中性纤维素酶 发酵条件 响应面分析法 

分 类 号：TQ925[轻工技术与工程—发酵工程]