超声波协同酶法提取浒苔多糖工艺的研究  

Extraction Process of Polysaccharides from Enteromorpha prolifera by Ultrasonic-Assisted Enzymatic Methods

作　　者：唐志红[1] 王硕[1] 鞠宝[1] 温少红[1] 秦松[2] 

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]中国科学院烟台海岸带研究所,山东烟台264003

出　　处：《烟台大学学报（自然科学与工程版）》2013年第4期303-306,共4页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省高等学校科技计划项目(J11LF71)

摘　　要：以浒苔为原料,采用超声波协同酶法提取多糖.在单因素试验的基础上采用正交试验优化浒苔多糖提取的工艺条件,并与热水浸提法进行比较.结果表明,浒苔多糖提取的最佳条件:超声时间20 min,料液比1∶50,温度50℃,酶用量3.0%,在此条件下重复试验,测得浒苔多糖的平均提取率为16.83%,同热水浸提法相比,多糖提取率明显提高,且提取时间大大缩短.Ultrasonic-assisted enzymatic methods are adopted to extract polysaccharides from Enteromorpha prolif- era. Based on single factor experiment results, orthogonal test L9 （34） is applied to optimize the extraction condi- tions. The results show that the optimum extraction conditions are as follows ： ultrasonic time 20 min, ratio of mate- rial to water 1：50, hydrolysis temperature 50 ℃, enzyme concentration 3%. Under the optimized conditions, the extraction rate reaches 16. 83%, which is higher than that of traditional hot water extraction, and the extraction time is significantly reduced. The optimized conditions can be used for the extraction of Enteromorpha prolifera pol- ysaccharides.

关 键 词：超声波 纤维素酶 浒苔 多糖 

分 类 号：S986.1[农业科学—捕捞与储运]