植物乳杆菌抗酸相关基因的序列特征性扩增区域(SCAR)标记  

Sequence-characterized Amplified Region(SCAR) Marker Linked to Acid Resistance-related Gene in Lactobacillus plantarum

作　　者：李凯[1] 朱成龙[1] 吕珍[1] 杨芮[1] 但霞[1] 刘树文[1,2] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100

出　　处：《食品科学》2013年第19期181-185,共5页Food Science

基　　金："十二五"国家科技支撑计划项目(2012BAD31B07);中央高校基本科研业务费专项(2109021104);国家现代农业产业技术体系建设专项(nycytx-30-ch-03)

摘　　要：以11株抗酸性和8株酸敏性植物乳杆菌为实验菌株,采用群体分组分析法(BSA)和随机扩增多态性DNA(RAPD)技术对55条引物进行筛选,并对基因池中扩增出差异性条带的引物进行单菌株验证,最终获得1个抗酸性RAPD标记:S116-680;将抗酸性RAPD特异片段回收,与pGEM-T Easy载体连接后测序;根据序列信息设计了一对特异性引物,该对引物能在11株抗酸性菌株中稳定扩增出一条680bp的特异性条带,命名为SA-680抗酸性相关标记。Totally 11 acid-resistant Lactobacillus plantarum strains and 8 acid-sensitive Lactobacillus plantarum strains were investigated, and random amplified polymorphic DNA （RAPD） and bulked segregate analysis （BSA） techniques were employed in this study. One RAPD marker S116-680 related to acid resistance in Lactobacillus plantarum was obtained through the selection from 55 random primers and the confirmation in a single Lactobacillus plantarum strain. The specific fragments were recovered and ligated into pGEM-T Easy vector, and then sequenced. According to the sequence information, a pair of specific primers （SA1/SA2） were designed, and SA1/SA2 with stable amplification of a 680bp specific band from 11 acid-resistant strains, was named as SA-680 acid resistance-related marker.

关 键 词：植物乳杆菌 抗酸相关基因 RAPD标记 SCAR标记 

分 类 号：Q933[生物学—微生物学] Q78