一株溶藻真菌的初步分离鉴定及其溶藻作用研究  

Isolation, identification and algicidal function of an algae-lysing fungus

作　　者：廖春丽[1] 吴创业[1] 郑瑞[1] 姬晓娜[1] 万亚涛[1] 单林娜[1] 

机构地区：[1]河南城建学院生物工程系,河南平顶山467044

出　　处：《广东农业科学》2013年第19期163-166,共4页Guangdong Agricultural Sciences

基　　金：河南省科技攻关重点项目(092102310069);河南城建学院校级项目(2010JYB010)

摘　　要：从水体中分离得到一株具有溶藻能力的真菌,命名为Am11。经形态学和18S rRNA序列对比分析,表明该菌株属于赤霉菌属(Gibberella)。研究了该菌株对湖泊中优势藻的溶藻效果,初步探讨了其溶藻方式及溶藻物质。结果表明,该菌株对小球藻、惠氏微囊藻、栅藻和蛋白核小球藻具有一定的去除效果,对4种藻叶绿素a的去除率分别为82.5%、64.9%、63.5%和85.4%;该菌株对蛋白核小球藻是间接溶藻,且溶藻因子是菌体胞外分泌的具有热稳定性的非蛋白类物质。Algicidal fungus Amll was isolated from water. According to morphology and 18S rRNA sequence analysis, algicidal fungus Aml 1 belonged to Gibberella. Algicidal efficiency of Aml 1 on dominant algae from lakes was studied, algicidal ways and algicidal matter were discussed. Results showed that Amll had certain removal effect on Chlorella, Microcystis wesenbergii, Scenedesmus and C. pyrenoidosa, the removal rate of chlorophyll a reached 82.5%, 64.9%, 63.5% and 85.4%, respectively. Amll dissolved C. pyrenoidosa indirectly, and the algicidal matter was extracellular, thermo-stable and non-protein substances secreted by algae-lysing fungus.

关 键 词：溶藻真菌Am11 分离鉴定 溶藻作用 

分 类 号：X172[环境科学与工程—环境科学]