Study on Improving the Internal Quality of Tobacco Stems by Using Pectinase  

利用果胶酶改善烟梗内在品质的研究(英文)

作　　者：何邦华[1] 申晓锋[1] 陈兴[1] 汪显国[1] 巩效伟[1] 李水荣[1] 朱勇[1] 邱昌桂 

机构地区：[1]红云红河烟草(集团)有限责任公司技术中心,云南昆明650231 [2]云南同创检测技术股份有限公司,云南昆明650106

出　　处：《Agricultural Science & Technology》2013年第9期1299-1302,1321,共5页农业科学与技术（英文版）

基　　金：Supported by the Special Fund of Hongyun Honghe Tobacco(Group)Co.,Ltd~~

摘　　要：[Objective] This study aimed to reduce the content of cell wall materials in tobacco stems and improve their internal quality. [Method] Pectinase was used to decompose cell wall materials in tobacco stems, followed by determination of the contents of four cell wall materials, six routine chemical components, as well as aroma constituents. [Result] Pectinase could effectively reduce the contents of cell wall materials in tobacco stems, with the largest decrease of 6.84%; after pectinase treatment,the content of reducing sugar in tobacco stems increased obviously, and the contents of total sugar, potassium ion, chloride ion and total nitrogen increased to varying degrees, of which the contents of potassium ion and reducing sugar displayed upward trends with the increase of pectinase concentration. Pectinase treatment significantly increased the contents of Maillard reaction products, with the most increase of 67.2%;the contents of carotenoid degradation products, phenylalanine degradation products and neophytadiene all increased to varying extents, and the contents of both Maillard reaction products and phenylalanine degradation products revealed ascending trends with the increase of pectinase concentration. [Conclusion] Pectinase treatment can effectively decompose cell wall materials in tobacco stems, improve routine chemical constituents, and increase the contents of aroma constituents.[目的]为了降低烟梗中细胞壁物质的含量,改善烟梗的内在品质。[方法]利用果胶酶处理烟梗,降解其细胞壁物质,并对烟梗中4种细胞壁物质、6种常规化学成分和致香成分进行测定。[结果]果胶酶可有效降低烟梗中细胞壁物质的含量,最高下降幅度为6.84%;果胶酶处理后,烟梗中还原糖含量明显升高,总糖、钾离子、氯离子和总氮也都有不同程度地升高,且随着果胶酶浓度的升高,钾离子、还原糖含量呈上升趋势;果胶酶处理可明显提高美拉德反应产物的量,最高可增加67.2%,类胡萝卜素降解产物、苯丙氨酸降解产物和新植二烯也都有不同程度地提高,且随着酶浓度的升高,美拉德反应产物和苯丙氨酸降解产物的量都有升高趋势。[结论]果胶酶处理烟梗可有效降解其细胞壁物质,改善常规化学成分,并能提高烟梗中致香成分的含量。

关 键 词：PECTINASE Tobacco stems Cell wall materials Routine chemical components Aroma constituents 

分 类 号：S572[农业科学—烟草工业]