分光光度法快速测定食品中硼砂的含量  

Rapid spectrophotometric determination of borax in foods

作　　者：胡颖[1] 汪倩[1] 谭量量[1] 金露[1] 赵澎涛 黄光荣[1] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018 [2]杭州天迈生物科技有限公司,浙江杭州310012

出　　处：《食品工程》2013年第3期44-47,共4页Food Engineering

基　　金：浙江省新苗人才计划项目(2012R409026);开放实验室项目(2012-78)

摘　　要：利用偶氮甲碱-H-钠盐与水杨醛结合,生成的甲亚胺H与硼形成黄色化合物,通过分光光度法可以简便地测定食品中硼砂的含量。研究结果表明,反应体系的最大吸收波长为413 nm,最佳显色条件为:H酸用量2.5 mL,水杨醛用量4.0 mL,反应温度25℃,反应时间15 min,终止反应液1.0 mol/L HCl用量1.2mL。在硼砂含量为0.0μg/mL^5.5μg/mL范围内,吸光度值与浓度呈线性,相关系数R2为0.995 1,方法检出限达0.27μg/mL,平均回收率为106.7%。A rapid spectrophotometric method was proposed to determine borax in foods, which was based on that a- zomethine-H reacted with salicylaldehyde to form methylenimine-H, and the latter reacted with boron to form a yellow complex. The yellow complex could be quantized by speetropotometrie method. The results showed that the optimum absorption wavelength of this yellow complex was 413 nm. The optimum reaction conditions were azomethine-H volume of 2.5 mL,salicylaldehyde volume of 4.0 mL, room temperature （25 ℃ ）, time of 15 min, termination solution of 1.2 mL 1.0N HC1. The linear relationship between absorbance and borax content was kept in the range of 0 μg/mL-5.5μg/mL with relationship index of R2=0.995 1. The detection limit of this proposed method was 0.27μg/mL and the average re- covery rate of 106.7%.

关 键 词：硼砂 分光光度法 H酸 快速检测 

分 类 号：TS207.51[轻工技术与工程—食品科学]