双孢菇废弃物菇柄多糖提取工艺研究  

Study on extraction technology of polysaccharides from Agaricus bisporus waste stipe

作　　者：侯传伟[1] 王安建[1] 李顺峰[1] 沈琪[1,2] 魏书信[1] 

机构地区：[1]河南省农科院农副产品加工研究所,河南郑州450002 [2]河南农业大学食品科学技术学院,河南郑州450002

出　　处：《食品工业科技》2013年第21期266-268,289,共4页Science and Technology of Food Industry

基　　金：河南省重大科技攻关计划项目(112101110300)

摘　　要：为扩大双孢菇废弃物菇柄的利用,以水为浸提溶剂,通过单因素实验研究了浸提温度、浸提时间、液固比、提取次数、乙醇用量和醇析时间对双孢菇废弃物菇柄多糖得率的影响,并采用正交实验对提取工艺进行优化。结果表明,多糖得率的主要影响因素及顺序为提取次数、浸提时间、浸提温度、液固比;在乙醇用量为2倍体积,醇析时间为2h时,最佳提取工艺参数为浸提温度85℃、浸提时间90min、液固比为15∶1、提取次数为3次,此时多糖得率为5.50%。In order to expend the uses of Agaricus bisporus waste stipe,water was taken as extraction solvent and the effects of extraction temperature, extraction time, ratio of liquid to solid, extraction times, ratio of ethanol to extract and ethanol precipitation time on the yield of polysaccharides from Agaricus bisporus waste stipe were investigated by the single factor experiments.Technology parameters were optimized by orthogonal test.The results showed that the degree of the factors significantly influencing the yield of polysaccharides was in order of extraction times, extraction time, extraction temperature, ratio of liquid to solid.The optimum extraction conditions were as follows：volume of ethanol to extract for precipitation was 2 times, precipitation time was 2h, extraction temperature 85~C,extraction time 90min, ratio of liquid to solid 15：1, extraction times 3.The yield of polysaccharides could be up to 5.50% under the optimum extraction conditions.

关 键 词：双孢蘑菇 废弃物菇柄 多糖 提取 优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]