双孢蘑菇W192菌株液体发酵培养的研究  

Study on Fermentation of Agaricus bisporus Strain W192

作　　者：曾志恒[1] 程翊[1] 曾辉[1] 丁中文[2] 戴建清[1] 

机构地区：[1]福建省农业科学院食用菌研究所,福建福州350014 [2]福建省农业科学院,福建福州350003

出　　处：《福建农业学报》2013年第8期763-769,共7页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2011R1022-4);闽发改投资([2012]931);晋安区科技计划项目([2012]11号)

摘　　要：采用响应面法对双孢蘑菇W192菌株液体发酵培养基及培养条件进行优化。通过单因子试验,确定最佳摇瓶培养基的碳源为玉米淀粉、氮源为黄豆粉、无机盐为K2HPO4和MgSO4·7H2O,最佳发酵条件为25℃,摇瓶转速180r·min-1,发酵时间7d,起始pH6.5。在此基础上,通过Plackett-Burman设计对影响其菌丝体生物量的8个考查因素进行评估并筛选出具有显著效应的玉米淀粉、黄豆粉和摇瓶转数为影响试验的3个重要因素。通过响应面分析法建立模型,确定发酵培养的最适培养基和培养条件:玉米淀粉43.67g·L-1,黄豆粉13.78g·L-1,KH2PO43.0g·L-1,MgSO4·7H2O 1.0g·L-1,发酵温度25℃,摇瓶转速200r·min-1,初始pH 6.5,发酵时间7d。在此条件下,每100mL菌丝体生物量可达2.58g,与初始培养条件下的菌丝体生物量相比提高了2.35倍。Culture medium and conditions for the fermentation of Agaricus bisporus strain W192 were optimized using the respond surface method . Determined by a single factor test ,the optimal carbon source for the mycelial development was cornstarch ;the nitrogen source ,soymeal ;and ,the inorganic salts ,K2 HPO4 and MgSO4 ？ 7H2 O . The fermentation was best carried out in a liquid medium with an initial pH of 6.5 in a flask held at 25 ℃ and shaken constantly at 180 r ？ min - 1 for 7 days . Using the Plackett-Burman design , the conditions for the A. bisporus mycelium biomass culture were evaluated .Cornstarch ,soymeal and flask-shaking speed were found to be the most prominent factors that affected the fermentation . Through the respond surface analysis ,a regression equation was obtained ,and the fermentation conditions optimized as cornstarch at 43.67 g ？ L - 1 ,soymeal at 13.78 g ？ L - 1 ,K2 HPO4 at 3.0 g ？ L - 1 ,MgSO4 ？ 7H2 O at 1.0 g ？ L - 1 ,incubation at 25 ℃ ,flask-shaking at 200 r ？ min - 1 ,medium initial pH at 6.5 ,and fermentation for 7 days . The resultant A. bisporus mycelium biomass reached a concentration of 2.58 g per 100 mL ,which was 2.35 times higher than the orginal conditions .

关 键 词：双孢蘑菇 W192 液体菌种 响应面 优化 

分 类 号：S646[农业科学—蔬菜学]