响应曲面法优化辣椒油树脂分离条件及分离产物的抗氧化活性测定  

Optimization of Molecular Distillation Separation Conditions for Capsicum Oleoresin by Response Surface Methodology and Antioxidant Activity of Its Separated Components

作　　者：陶红[1] 蒋林[1] 郑锡康[1] 钟文俊[1] 胡继藤[1] 王晓娟[1] 杜卓[2] 陈金龙[1] 

机构地区：[1]中山大学药学院生药与天然药物化学实验室,广东广州510006 [2]佛山科学技术学院医学院药学系,广东佛山528000

出　　处：《食品科学》2013年第20期87-93,共7页Food Science

摘　　要：采用分子蒸馏技术对辣椒油树脂进行分离纯化,考察蒸馏温度、蒸馏压力、刮膜转数对辣椒碱类物质和辣椒红色素分离的影响。应用响应面法对其工艺参数进行优化,通过Box-Behnken试验设计,研究各因素及其交互作用对响应值的影响,建立了二次多项回归方程的预测模型,确定了最佳的纯化工艺条件为蒸馏温度144℃、蒸馏压力9.5Pa、刮膜转数313r/min。在此条件下,辣椒碱类物质的含量为75.30%,辣椒红色素的色价为19.84。同时,通过清除DPPH自由基抗氧化活性实验测定分离得到的辣椒碱类物质具有良好的抗氧化活性。Molecular distillation （MD） was used to purify capsicum oleoresin, and three important process parameters such as distillation temperature, distillation pressure and wiper rolling speed were optimized by response surface methodology for better separation between capsaicinoids and capsicum red pigment. Individual variables and their interactions on the response values capsaicinoid concentration and color value were explored according to Box-Behnken design. A mathematical quadratic polynomial regression equation was established. The optimal process conditions for distillation temperature, distillation pressure and wiper rolling speed were 144 ℃, 9.5 Pa and 313 r/min, respectively. Under these conditions, the capsaicinoids concentration was increased to 75.30%, and the color value of capsicum red pigment separated was 19.84. DPPH radical scavenging assay showed that the capsaicinoids separated had good antioxidant activity.

关 键 词：辣椒碱 分子蒸馏 响应曲面法 抗氧化活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]