抑制氧化罗丹明B光度法测定贝类中牛磺酸含量  

Spectrophotometric Determination of Taurine in Shellfish Using Inhibition of Rhodamine B Oxidation

作　　者：李咏梅[1,2] 李人宇[3] 施鹏飞[2] 

机构地区：[1]淮海工学院江苏省海洋生物技术重点建设实验室,江苏连云港222005 [2]淮海工学院化学工程学院,江苏连云港222005 [3]连云港师范高等专科学校,江苏连云港222006

出　　处：《食品科学》2013年第20期117-120,共4页Food Science

基　　金：国家自然科学基金青年科学基金项目(21101069);淮海工学院江苏省海洋生物技术重点建设实验室研究基金项目(2012HS007)

摘　　要：在pH11.0氨-氯化铵缓冲溶液中,痕量牛磺酸能抑制高锰酸钾氧化罗丹明B的反应,使体系吸光度明显减小,据此建立了分光光度法测定牛磺酸的新方法。最大褪色波长为760nm,体系的褪色程度与牛磺酸质量浓度在0～100μg/L范围内呈线性关系,表观摩尔吸光系数为1.07×106L/(mol·cm),检出限为2.25μg/L。方法用于测定缢蛏、文蛤、四角蛤蜊和菲律宾蛤仔中牛磺酸含量,结果与高效液相色谱法一致,相对标准偏差为0.76%～1.08%(n=6),回收率为96.93%～102.40%。A novel method for the spectrophotometric determination of taurine was developed. The method was based on the inhibitory effect of trace taurine on the reaction of potassium hypermanganate oxidizing rhodamine B in ammonium chloride/ammonia buffer solution at pH 11.0, resulting in an obvious decrease in the absorbance. The maximum detection wavelength for fading reaction was 760 nm. A linear relationship was observed between the fading grade and the concentration of taurine in the range of 0–100 μg/L with an apparent molar absorption coefficient of 1.07 × 106 L/（mol？cm） and the detection limit was 2.25 μg/L. This method was applied to determine taurine in Sinonovacula constricta, Meretrix meretrix, Mactra veneriformis and Ruditapes philippinarum. The results revealed good agreement with those obtained by high performance liquid chromatography （HPLC）. The relative standard deviations were 0.76%–1.08% （n = 6）, and the average recovery rates were 96.93%–102.40%.

关 键 词：牛磺酸 分光光度法 罗丹明B 贝类 

分 类 号：TS207.3[轻工技术与工程—食品科学]