三聚氰胺胶体金免疫层析方法的建立  

Development of Lateral Flow Assay for Melamine Detection Based on Colloidal Gold

作　　者：夏骏[1,2] 赖卫华[1] 涂继平 徐国茂 张新宇 冯荣华[1] 衷平海[1] 何小立[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]江西省兽药饲料监察所,江西南昌330029 [3]江西中德生物工程有限公司,江西南昌330047

出　　处：《食品科学》2013年第20期139-143,共5页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAK10B01)

摘　　要：建立了一种快速检测三聚氰胺的胶体金试纸条的方法,其中包括胶体金的制备、金标抗体的制备、试纸条的组装和测试等步骤。结果表明:三聚氰胺快速检测试纸条的检测限为10 g/L,检测时间为10min,可实现原料乳不经处理直接上样,是一种简便准确的快速检测方法。A method for rapid detection of melamine by colloidal gold strip was introduced. Four steps were involved in the process of preparing the strip, including production of colloidal gold, manufacture of immunogold, installation and analysis of the strip. With the strip, 10 μg/L of melamine was detected in less than 10 min. Fresh milk could be detected directly without pretreatment, so it provided a simple, accurate and rapid method for on-site testing of melamine.

关 键 词：胶体金 三聚氰胺 免疫层析 检测 

分 类 号：TS207.7[轻工技术与工程—食品科学]