甘蔗固有多糖提取分离及单糖组成分析  

Isolation and Monosaccharide Composition Analysis of Indigenous Sugarcane Polysaccharide

作　　者：班甲[1] 陈骏佳[1] 胡雪梅[1] 徐晓燕[1] 付尽国[1] 孟飞[1] 王帆[1] 

机构地区：[1]广州甘蔗糖业研究所、广东省甘蔗改良与生物炼制重点实验室,广东广州510316

出　　处：《食品科学》2013年第20期182-185,共4页Food Science

基　　金：科技部科研院所技术开发研究专项(2011EG111248)

摘　　要：采用乙醇沉淀法提取分离4种甘蔗清汁中的固有多糖,用苯酚-硫酸法测定多糖含量,酸水解后用气相色谱法对固有多糖的单糖成分进行初步分析。结果表明:与实验室制取的甘蔗清汁相比,糖厂清汁中固有多糖含量明显偏高;4种甘蔗清汁中固有多糖的单糖组成相同,共包括:核糖、鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖7种单糖;由各单糖组分推测清汁中固有多糖主要来源于甘蔗纤维中的半纤维素与纤维素;4种固有多糖中各单糖组分相对含量有明显差异。Indigenous sugarcane polysaccharide （ISP） was obtained from four clarified juice from different cultivars of sugarcane by ethanol precipitation. The ISP content was determined using phenol-sulfuric acid method and the ISP composition after acid hydrolysis was analyzed by gas chromatography. The results showed that the ISP content in the clarified sugarcane juice from industrial production （a hybrid from three cultivars） was higher than in the laboratory prepared ones （from three other cultivars, respectively）. The ISP monosaccharide composition of all the clarified sugarcane juices was the same, including ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose. The above results suggested that the ISP in the clarified sugarcane juice mainly derived from the hemicelluloses and celluloses in the sugarcane fiber. The relative contents of the monosaccharides had a significant difference among these four sugarcane juices.

关 键 词：甘蔗 固有多糖 分离 单糖组成 

分 类 号：TS241[轻工技术与工程—制糖工程]