绍兴市售酸奶中乳酸菌的分离鉴定及耐药性分析  

Isolation of the Lactic Acid Bacteria and Analysis of the Antibiotic Resistance from the Yogurt Sold in Shaoxing City

作　　者：陈燃[1] 段会会[1] 陈慧来[1] 金丹[1] 胡春霞[1] 

机构地区：[1]绍兴文理学院元培学院生命科学系,浙江绍兴312000

出　　处：《安徽农业科学》2013年第21期9070-9071,共2页Journal of Anhui Agricultural Sciences

基　　金：绍兴市大学生科技创新项目

摘　　要：[目的]从绍兴市售酸奶中分离并鉴定乳酸菌,研究乳酸菌分离株对6种抗生素的耐药性。[方法]利用MRS培养基通过稀释法分离酸奶样品中的乳酸菌并进行菌落计数,对分离菌株进行革兰染色和生化反应鉴定,同时采用纸片扩散法进行耐药性分析。[结果]从15种绍兴市售酸奶样品中共分离出31株菌,生化反应鉴定为嗜酸乳杆菌(6株)、保加利亚乳杆菌(7株)、嗜热链球菌(11株)和双歧杆菌(7株);活菌计数结果显示,4份样品(26.7%)中乳酸菌计数低于国家标准,11份样品(73.3%)中活菌计数符合国家标准规定;耐药性试验结果显示,31株乳酸菌对4种抗生素均有一定程度的耐受性,且90.3%的菌株具有多重耐药性,能耐受2～3种抗生素。[结论]绍兴市售酸奶中乳酸菌已具有一定程度的耐药性,对酸奶产品进行耐药性监测和安全评价已势在必行。[ Objective ] To analyze the antimicrobial susceptibility of lactic acid bacteria isolated from yogurt sold in Shaoxing to erythromycin, compound sulfamethoxazole, cefotaxime sodium, vancomycin, gentamicin and clindamycin. [ Method ] Lactic acid bacteria were recovered and counted from yoghourt by plate count method. The recovered isolates were characterized by Gram＇s strain, biochemical test, antimicrobial susceptibility test. [ Result] 31 probiotic isolates were recovered from 15 yoghourt products, which was identified as Lactobacillus acidophilus （ 6 strains ）, Lactobacillus bulgaricus （ 7 strains ）, Streptococcus thermophilus （ 11 strains）, Bifidobacterium （ 7 strains ） ; 4 （ 26.7 % ） products showed a lower probiotic quantity than 1 ~ 106 CFU/ml of the national standard, and 11 （73.3%） products measured up the national stand- ard; drug resistance test results showed that the 31 isolates were resistant to 4 types of antibiotics, and 28 isolates （90.3%） were of multiple resistance, and resistant to 2 - 3 types of antibiotics. [ Conclusion ] The antimicrobial resistances of lactic acid bacteria had been developed, and monitoring their antimicrobial resistance and evaluatinz their safety should be imperative.

关 键 词：酸奶 乳酸菌 分离鉴定 耐药性 

分 类 号：R155.57[医药卫生—营养与食品卫生学]