高效液相色谱法测定啤酒中微量甲醛方法研究  

A Study of Determination of Micro-quantity Formaldehyde in Beer by HPLC

作　　者：彭科怀[1] 陈江[1] 赵年华[1] 伍红[2] 

机构地区：[1]四川省卫生防疫站,成都610031 [2]西南民族学院,成都610041

出　　处：《西南民族学院学报（自然科学版）》2000年第2期159-162,共4页Journal of Southwest Nationalities College(Natural Science Edition)

摘　　要：HPLC测定啤酒中微量甲醛方法是将甲醛用水蒸馏后用 0 .2 % 2 .4 二硝基苯肼吸收 ,氯仿提取 ,HPLC测定 ,根据Rt定性、峰高定量 .此法效果好 ,灵敏度高 ,准确性高 .The procedure of determining micro quantity formaldehyde in beer by HPLC involves distilling formaldehyde from the beer sample firstly, then being absorbed by 2,4 dinitrophenylhydrazone, extracted by chloroform,and finally measured through HPLC. The study shows that this method is accurate and sensitive with the detection limit ranging from 0.0125 μg to 0.10μg.

关 键 词：HPLC 甲醛 2.4-二硝基苯肼 高效液相色谱法 啤酒 

分 类 号：TS262.5[轻工技术与工程—发酵工程] O657.72[轻工技术与工程—食品科学与工程]