乳酸菌中糖诱导的基因表达研究进展  

Advance in Lactic Acid Bacteria Sugar-induced Gene Expression

作　　者：曾珠[1] 陈丽丽[1] 左芳雷[1] 赵伟[1] 陈尚武[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国生物工程杂志》2013年第10期131-137,共7页China Biotechnology

基　　金：国家自然科学基金资助项目(31071507)

摘　　要：乳酸菌是指一类能发酵碳水化合物产生乳酸的革兰氏阳性菌的通称,常用于表达外源蛋白以及作为药物分子的载体。乳酸菌中异源基因的表达通常包含组成型启动子和诱导型启动子,国内外学者研究了许多诱导型的表达系统,包括Nisin诱导、温度诱导、糖诱导等。糖在乳酸菌工业中具有非常重要的作用,同时糖诱导的表达系统具有许多优势,因此国内外对糖诱导的基因表达进行了深入的研究。现主要从糖代谢的机理、糖诱导的基因表达等方面(包括乳糖、木糖、麦芽糖等)对其进行综述,旨在为乳酸菌糖诱导型表达外源蛋白研究提供资料。Lactic acid bacteria is a kind of gram-positive bacteria which can ferment carbohydrate to produce lactic acid, and it is often used for expressing heterologous proteins as well as carrier for drug molecules. Lactic acid bacteria heterologous gene express usually includes constitutive promoters and induced promoters, many induced express systems have been elucidated at home and abroad such as Nisin inducing, temperature inducing, sugar inducing and so on. Sugar plays a very important role in Lactic acid bacteria industry and sugar-induced express systems have so many advantages that they have been thoroughly studied domestic and overseas. The mechanism of sugar metabolism, sugar-induced gene express（including lactose, xylose, maltose etc.） were reviewed to provide reference for Lactic acid bacteria sugar-induced expressing heterologous proteins.

关 键 词：糖诱导 启动子 乳酸菌 

分 类 号：Q786[生物学—分子生物学]