复合酶法提取蛹虫草活性多糖的工艺优化研究  

Extraction processing optimization of polysaccharides from Cordyceps militaris L.Link by compound enzyme

作　　者：钟旭美[1] 陈铭中[1] 陈丽琼[1] 

机构地区：[1]阳江职业技术学院生命科学与技术系,阳江529566

出　　处：《食品科技》2013年第10期210-213,共4页Food Science and Technology

基　　金：国家质检总局科技计划项目(2009IK165);贵州省高层次人才科研条件特助经费项目(TZJF-2007-18)

摘　　要：将木瓜蛋白酶和纤维素酶按一定比例制成复合酶,以单因素实验为基础,采用正交实验对影响蛹虫草多糖的提取的因素:提取温度、提取时间及料液比进行工艺优化,得出了蛹虫草多糖提取的最佳工艺:水浸提温度为80℃、时间2 h、料液比为1:25,为蛹虫草功能性食品开发提供理论依据。This research aimed to optimize the extraction process of polysaccharides from Cordyceps militaris L. Link by the compound enzyme made from papaya protease and cellulase in certain proportion. Using the orthogonal experiment with single factor experiment as the foundation, the influencing factors of polysaccharide extracted from Cordyceps militaris L. Link was discussed as follow： extraction temperature, extraction time and ratio of solid to liquid. The optimum extraction process of polysaccharides from Cordyceps militaris L. Link was as follow： water extracted temperature 80 ℃, time 2 h, ratio of solid to liquid 1：25. The result can offer a theoretical basis for functional food development of Cordyceps militaris L. Link.

关 键 词：蛹虫草 活性多糖 提取工艺 

分 类 号：R284.2[医药卫生—中药学]