去神经支配大鼠海马内MTSS1蛋白的表达变化  

The expression change of MTSS1 protein in the deafferented rat hippocampus

作　　者：赵荷艳[1] 金国华[1] 王海琴[1] 李浩明[1] 邹琳清[1] 张蕾[1] 秦建兵[1] 

机构地区：[1]南通大学医学院人体解剖学系,江苏省神经再生重点实验室,南通226001

出　　处：《南通大学学报（医学版）》2013年第5期356-358,共3页Journal of Nantong University(Medical sciences)

基　　金：南通大学自然科学基金资助项目(10Z059);江苏省高校优势学科建设工程资助项目(PAPD)

摘　　要：目的 :探讨切割穹窿海马伞去神经支配大鼠海马内(metastasis suppressor 1,MTSS1)蛋白的表达变化。方法:SD大鼠随机分成正常组和切割穹窿海马伞后1、3、5、7、14 d组,采用Western Blot法检测穹窿海马伞切割后海马内MTSS1蛋白表达水平的变化。结果:海马内MTSS1蛋白的相对表达量在切割后1 d开始升高(P<0.05),3 d继续升高(P<0.01),5 d达到最高水平(P<0.01),7 d开始下降(P<0.01),14 d时基本恢复至正常水平(P>0.05)。结论:切割穹窿海马伞去神经支配后海马内MTSS1蛋白表达水平明显上调,可能与海马神经再生过程中神经干细胞向神经元的分化有关。Objective： To observe the metastasis suppressor I（MTSS1） protein expression in hippocampus of fimbria-fornix transected rats. Methods： SD rats were randomly divided into normal control group and fimbria-fornix transected 1st , 3rd , 5th, 7th and 14th day group respectively. Western Blot was used to analyze the expression of MTSS1 protein in hippocampus between the fimbria-fornix transected rats and normal ones. Results： The relative expression level of MTSS1 protein began to rise on the 1st day（P〈0.05）, continued to increase on the 3rd day（P〈0.01）, and reached its highest level on the 5th day （P〈0.01）. Then it began to decline on the 7th day（P〈0.01） and returned to normal levels on the lgth day（P〉0.05）. Conclusion： The up-regulated MTSS1 protein in the fimbria-fornix transected hippocampus may be related to neuronal differentiation of hippocampal neural stem cells.

关 键 词：METASTASIS SUPPRESSOR 1 穹窿海马伞切割 海马 神经再生 大鼠 

分 类 号：R361[医药卫生—病理学]