芦荟黄酮体内抗氧化活性及对CAT mRNA表达的影响  

Effect of Aloe Flavonoids on the Anti-oxidant Activity and CAT mRNA Expression in Vivo

作　　者：王文君[1] 蔡教英[1] 蒋艳[1] 杨武英[1] 徐明生[1] 上官新晨[1] 

机构地区：[1]江西农业大学江西省天然产物与功能食品重点实验室,南昌330045

出　　处：《中国食品学报》2013年第10期13-18,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31160319);江西省科技厅重点项目(2009BGA01900);江西省自然科学基金项目(2009GZN0014,2008GQN0020);江西省教育厅科学研究项目(GJJ11403)资助

摘　　要：目的:研究芦荟黄酮体内抗氧化活性及对CAT mRNA表达的影响。方法:采用超声波萃取,真空浓缩,冷冻干燥,用聚酰胺树脂纯化制备黄酮。用芦荟黄酮对高脂血症小鼠进行干预后,测定肝脏和脂肪组织T-AOC、CAT、GSH-Px、MDA的含量,以此评定其体内抗氧化功能。采用半定量PCR法测定肝脏和脂肪组织中CAT mRNA表达水平,从分子水平探讨芦荟黄酮抗氧化的机制。结果:芦荟黄酮能显著提高肝脏和脂肪组织中TAOC、CAT、GSH-Px的活性,抑制MDA的水平;同时可显著增强肝脏和脂肪组织中CAT mRNA的表达量。结论:芦荟黄酮具有显著的抗氧化和提高CAT mRNA表达水平的作用。Objective： To study the anti-oxidant activty and CAT mRNA expression of Aloe flavonoids in vivo. Methods： Flavonoids were preperaed with ultrasonic extraction, freeze-drying, vaccurn concentration and polyamide polymer purification. Then the flavonoids were feed to the hyperlipemia mice which induced with high fat emulsion for tour weeks, and the T-AOC, CAT, GSH-Px, MDA concentrations were detected in the liver and adipose to evaluate the an- ti-oxidant activity; meanwhile the CAT mRNA expressions were investigated by semi-quantitative PCR in the liver and the adipose to explore the anti-oxidant mechanism of the Aloe. Results： Aloe flavonoids could significantly enhance the T-AOC, CAT, GSH-Px activities and decrease the MDA level, and improve the CAT mRNA expression levels in the liver and adipose. Conclusion： Aloe flavonoids had good anti-oxidant effceets and improved the CAT mRNA expression in vivo.

关 键 词：芦荟 黄酮 抗氧化 CAT 表达 

分 类 号：TS201.2[轻工技术与工程—食品科学] R285[轻工技术与工程—食品科学与工程]