弹性蛋白酶提取牛蛙皮胶原蛋白的工艺优化  

Optimized Extracting Conditions of Collagen from Bullfrog Skin with Elastase

作　　者：杨远帆[1,2] 李杏[1] 陈申如[1] 伍菱[1] 倪辉[1,3,2] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]厦门市食品与生物工程技术研究中心,福建厦门361021 [3]福建省高校食品微生物与酶工程技术研究中心,福建厦门361021

出　　处：《中国食品学报》2013年第10期66-72,共7页Journal of Chinese Institute Of Food Science and Technology

摘　　要：牛蛙皮是一种优质的胶原蛋白资源。为了克服用普通蛋白酶提取胶原蛋白得率低的问题,采用弹性蛋白酶处理牛蛙皮,使其胶原蛋白高效溶出,从而提高牛蛙皮胶原蛋白的提取率。以胶原蛋白提取率为指标,用单因素试验方法研究温度、pH值、反应时间等因素对提取效果的影响;用中心组合试验方法优化料水比和酶的添加量。试验结果表明,用弹性蛋白酶提取牛蛙皮胶原蛋白的最佳条件是:pH 8.0,料液比1∶10,温度55℃,加酶量45.22 U/g,时间5 h。在此条件下胶原蛋白最大提取率为47.7%。用弹性蛋白酶提取牛蛙皮胶原蛋白具有良好的应用前景。The bullfrog skin is a new kind of high quality collagen resource. In order to solve the problem of low extraction rate of collagen with commercial enzymes, elastase is used to treat bullfrog skin for efficient dissolving the collagen. For the development of the enzymatic process with elastase, the effects of the main parameters, including extracting temperature, pH value, hydrolysis time were investigated with single factor experiment, and the dosage of elastase, ratio of solid to liquid were investigated with response surface methodology （RSM）. The optimum condition is： pH 8.0, solid-liquid ratio 1：10, hydrolysis temperature 55 ℃, concentration of elastase 45.22 U/g, treatment time 5 h. Under this condition, extraction ratio of collagen attained 47.7% from bullfrog skin. The results indicate this process has a good practical application in the production of bullfrog skin collagen.

关 键 词：牛蛙皮 胶原蛋白 弹性蛋白酶 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]