梅利斯丛梗孢酵母发酵生产赤藓糖醇的条件优化  

Optimization of Fermentation Conditions for Erythritol Production by Moniliella mellis and Wickerhamomyces anomalus

作　　者：蔡威[1] 张建志[1] 江正强[2] 闫巧娟[1] 

机构地区：[1]中国农业大学工学院,北京100083 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2013年第21期259-263,共5页Food Science

摘　　要：研究梅利斯丛梗孢酵母(Moniliella mellis)耦合异常威克汉姆酵母(Wickerhamomyces anomalus)发酵生产赤藓糖醇。通过单因素试验优化梅利斯丛梗孢酵母C-703产赤藓糖醇的发酵条件,得到最佳摇瓶发酵条件:培养基为葡萄糖250g/L和酵母提取物17.5g/L,初始pH5.5,250mL摇瓶装液20mL,温度28℃。在该条件下,发酵6d赤藓糖醇产量为84.6g/L。梅利斯丛梗孢酵母在5L发酵罐补料发酵10d,赤藓糖醇产量达114g/L。进一步将发酵液接入异常威克汉姆酵母C-430进行二次发酵,除去绝大部分副产物丙三醇,再经精制和结晶得到赤藓糖醇晶体,转化率为93.2%。结果表明双酵母耦合发酵生产赤藓糖醇具有较大的应用前景。Erythritol production by sequential fermentation with Moniliella mellis and Wickerhamomyces anomalus wasinvestigated in this report. The optimal fermentation conditions for Moniliella mellis C-703 were obtained by one-factor-ata-time design as follows： the fermentation medium containing glucose 250 g/L and yeast extract 17.5 g/L at initial pH 5.5, 20 mLof the medium in a 250-mL flask and 28 ℃. After 6 d of fermentation under these conditions, the yield of erythritol was 84.6 g/L.This process was followed by fed-batch fermentation with the same strain in a 5-L fermentor for 10 d, yielding 114 g/L oferythritol fermented broth. The second fermentation was performed by inoculation of Wickerhamomyces anomalus C-430.As a result, most of the byproduct glycerol was removed and erythritol crystals were obtained with a yield of 93.2% afterrefining and crystallization. Hence sequential fermentation with Moniliella mellis and Wickerhamomyces anomalus has apromising prospect for the application in erythritol production.

关 键 词：梅利斯丛梗孢酵母 异常威克汉姆酵母 赤藓糖醇 发酵 

分 类 号：TS202.3[轻工技术与工程—食品科学]