阿魏酸酯酶的制备及在肉鸡饲料中的应用  

Preparation of feruloyl esterase and application in feed of broiler

作　　者：杨道秀[1] 许文江[2] 王林林[1] 蔡婀娜[1] 李夏兰[1] 

机构地区：[1]华侨大学化工学院,福建厦门361021 [2]福建省亚热带植物研究所,福建厦门361002

出　　处：《化工进展》2013年第11期2685-2690,共6页Chemical Industry and Engineering Progress

基　　金：福建省科技重点项目(2011N0020);厦门市集美区科技计划项目(2024C 01)

摘　　要：为了降低饲料中非淀粉多糖质量抗营养因子,提高肉鸡对饲料养分的利用,本文研究了固态发酵阿魏酸酯酶的工艺及其对饲料养分利用率的影响。结果表明:在麦麸与麦糟质量比为2∶8、蛋白胨与酵母粉质量比为2∶4、含水量为40%、接种量为10%、培养时间为6天、培养温度为33℃条件下,阿魏酸酯酶的酶活达到了29.49 U/g,较未优化前提高了5.11倍。将浓缩后的酶液与溢多酶AF831添加入肉鸡饲料中,发现添加溢多酶及阿魏酸酯酶可以提高干物质、粗蛋白、粗灰分、酸性洗涤纤维等利用率,但作用不明显,对中性洗涤纤维改善效果极显著(P<0.01)。To reduce non-starch polysaccharides anti-nutritional factor in feed and promote intake of nutrient,the solid-state fermentation technology of feruloyl esterase （FAE） and the influence on nutrient utilization rate were studied. Under optimized conditions of 2∶8 of the ratio for wheat bran and brewers’ spent grain （BSG）,2∶4 of the ratio for peptone and yeast,40% of water content,10% of inoculum,6 days of incubation and 33 ℃ of incubation temperature,the activity of FAE was improved to five times compared to unoptimized conditions and the maximum activity was 29.49 U/g. The combination of FAE and Yiduoli enzyme was mixed in feed for broiler. FAE and Yiduoli enzyme could improve the utilization of dry matter,crude protein,crude ash,acidic detergent fiber,but the improvement was limited. The utilization of neutral detergent fiber increased markedly （P〈0.01）.

关 键 词：阿魏酸酯酶 溢多酶 麦糟 养分利用率 固态发酵 

分 类 号：S816.7[农业科学—饲料科学]