稻草秆腐解菌产酶条件优化及菌种鉴定  

The Optimum Condition for Enzyme Production in Straw Decomposition and Strain Identification

作　　者：魏赛金[1,2] 王世强[2] 胡殿明[2] 倪国荣[1] 涂国全[2] 潘晓华[1] 

机构地区：[1]江西农业大学作物生理生态与遗传育种教育部重点实验室/江西省作物生理生态与遗传育种重点实验室,江西南昌330045 [2]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《江西农业大学学报》2013年第5期1042-1047,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家“十二五”科技支撑计划项目(2011BAD16B04、2012BAD14B14);江西农业大学自然科学自由申请项目(2010)

摘　　要：以促进稻草秆腐熟速度为目的,分别研究了速效碳源、速效氮源和接种量对真菌F23、产纤维素酶和半纤维素酶能力的影响,并对该菌种进行鉴定。结果表明:蔗糖和尿素是真菌F23快速启动稻草秆降解的首选速效碳源和氮源。通过正交试验筛选适合F23产酶的最佳培养条件为1.5%蔗糖、1.5%尿素和7.5%接种量。根据该菌株的形态特征、培养特征的观察,ITS序列分析,鉴定菌株F23为黑曲霉。Starting from promotion of the decomposition rate of straw stalks,the impacts of availabe carbon and avilable nitrogen sources and inoculum amount on cellulase and hemicellulase producing capacity of F23 were studied.The results showed that sucrose and carbamide were the preferred carbon and nitrogen sources for straw-degradation.The optimal culture conditions for enzyme production of the strain F23 were screened through the orthogonal test.The results illustrated that the optimum conditions for fermentation were 1.5％ sucrose,1.5％ carbamide and 7.5％ inoculum amount.The strain F23 was identified as Aspergillus niger according to its morphological,cultural characteristics and sequence analysis of the ITS region.

关 键 词：秸秆 纤维素酶 半纤维素酶 正交试验 菌种鉴定 

分 类 号：Q949.327.1[生物学—植物学] S182[农业科学—农业基础科学]