抑郁症多巴胺受体DRD_2基因突变检测  

Screening for mutations of DRD_2 gene in depressive patients

作　　者：刘兆云[1,2] 龙逢[1,2] 刘苗[1,2] 陈星[1] 常学润[3] 陈刚[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062 [2]济南大学山东省医学科学院医学与生命科学学院 [3]山东省精神卫生中心

出　　处：《精神医学杂志》2013年第5期321-325,共5页Journal of Psychiatry

基　　金：国家自然科学基金项目(编号:30440042;30770780);山东省科技发展计划项目(编号:2011GSF11821);山东省自然科学基金重点项目(编号:Z2004C10)

摘　　要：目的对抑郁症患者DRD2基因外显子进行突变筛查。方法对DRD2基因的9个外显子设计合成15对引物,将238例抑郁症患者分为3组,分别构建3个DNA混合样本(96例、96例与46例)。将1 095例正常对照者分为两组,分别构建2个DNA混合样本(95例和1 000例)。将上述5个DNA混合样本作为DNA模板,先对其进行聚合酶链扩增,用Light Scanner高分辨率熔解曲线系统(HRM)筛查突变并确定TC值;对PCR产物稀释40倍后作为模板进行COLD-PCR扩增后再进行HRM突变检测。结果对患者组与两个对照组熔解曲线逐一对比后未发现患者DRD2基因外显子存在突变。结论抑郁症的病因并非源于DRD2基因外显子的突变。Objective To screen the mutations of DRD2 gene in depressive patients.Methods 15 pairs of primers covering all the 9 exonic sequences of DRD2 gene were applied to amplify the 3 DNA pools of depressive patients （n =96,96 and 46 respectively） and 2 DNA pools of healthy controls （n =95 and 1 000 respectively）.PCR products were scanned with high resolution melting curve analysis （HRM） and the critical melting temperature （Tc） was determined.PCR products were diluted 40 times as template for COLD-PCR amplification,then HRM was carried out again to compare the curve differences between depressive cases and healthy controls.Results Melting curve comparison did not reveal any mutation in depressive patients.Conclusion The etiology of depression is not associated with exonic mutations of DRD2 gene.

关 键 词：抑郁症 DRD2 基因 突变 高分辨率熔解曲线分析 

分 类 号：R749.4[医药卫生—神经病学与精神病学]