牛玻璃酸酶酶学特性的研究  

Study on enzymatic characteristics of hyaluronidase from bovine testicular

作　　者：杨萌萌[1] 韩玲[1] 李儒仁[1] 余群力[1] 左秀峰 韩广星 郭兆斌[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]山东绿润食品有限公司,山东临沂276000

出　　处：《食品工业科技》2013年第22期161-164,共4页Science and Technology of Food Industry

基　　金：国家现代农业(肉牛牦牛)产业技术体系项目资助(CARS-38);国家公益性行业(农业)科研专项经费资助(201203009)

摘　　要：以牛睾丸为原料，对纯化后玻璃酸酶的酶学性质进行研究。结果表明，牛玻璃酸酶水解透明质酸钠的最适pH和温度分别为4．5、37℃，在4-45℃酶活稳定性较好。Nacl和BSA浓度分别为0．2、0．45mol/L时具有较高的酶活力。金属离子抑制该酶活力的顺序为，Fe3＋〉Mn2＋〉Zn2＋〉Al3＋〉Ca2＋〉K＋〉Mg2＋。硫酸软骨素A浓度为1．2mg／mL时，酶活损失31．08％。硫酸软骨素B浓度为0．012mg／mL时，酶活增加1．36％。硫酸软骨素c浓度为1．2mg／mL时，酶活损失45．07％。肝素在0．012mg/mL时对酶存在抑制作用。人类血清蛋白在0．012mg/mL时可增加1．05％的酶活。半胱氨酸使酶失活。The enzyme used in this study was a partially purified sample of hyaluronidase,extracted and purified from bovine testicular. Using sodium hyaluronate as substrate,the pH optimum for the enzyme was pH4.5 and maximum activity was obtained at 37℃. The enzyme also required NaCI and BSA at a concentration of 0.2mol/L and 0.45mol/L in the final incubation mixture for optimum activity. The hyaluronidase from bovine testicular was shown to be heat instable,and inhibited by metal ions,the inhibiting capacity was Fe3＋〉Mn2＋〉Zn2＋〉Al3＋〉Ca2＋〉K＋〉Mg2＋. Chondroitin-sulphate A and C was inhibitor of bovine hyaluronidase,causing up to 31.08% and 45.07% inhibition at 1.2mg/mL concentration. The enzyme activity was enhanced 1.36% and 1.05% by chondroitin-sulphate B and human serum protein at 0.012mg/mL concentration,respectively. Heparin inhibited enzyme activity at 0.012mg/mL concentration. Cysteine completely inhibited enzyme activity.

关 键 词：牛睾丸 玻璃酸酶 酶学特性 

分 类 号：TS251.95[轻工技术与工程—农产品加工及贮藏工程]