水溶性可得然寡糖的制备及其活性研究  

Preparation of Water-soluble Curdlan Oligose and Determination of Its Activity

作　　者：王英杰[1] 赵双枝[2] 苏理[2] 兰文忠 

机构地区：[1]齐鲁工业大学食品与生物工程学院,山东济南250353 [2]山东省食品发酵工程重点实验室,山东省食品发酵工业研究设计院,山东济南250013

出　　处：《现代食品科技》2013年第11期2735-2741,共7页Modern Food Science and Technology

摘　　要：研究了在碱性条件下,利用双氧水均相氧化降解可得然胶,制备低分子量水溶性可得然寡糖的工艺条件。通过单因素试验对氢氧化钠浓度、双氧水加量、反应温度、反应时间等影响降解的条件进行了优化,最终确定了降解的最佳工艺条件是氢氧化钠的浓度为2 mol/L、双氧水加量为1.5%(V/V)、降解温度为40℃、降解时间为48 h。利用纸层析和红外光谱对降解产物进行表征,结果表明降解后产品的化学结构未发生改变。进一步研究了产物可得然寡糖体内抗肿瘤、对竹节虾生长性能影响及抑菌试验,结果表明:可得然寡糖具有一定的抑瘤效果,小鼠实验表明,剂量为50 mg/kg体重时,抑瘤率可达48.73%;竹节虾饲料中添加可得然寡糖可提高其存活率,添加量为400 mg/kg时,酚氧化酶活力最高,达到2.96 U/mg;可得然寡糖对金黄色葡萄球菌具有一定的抑菌效果,对大肠杆菌具有较微弱的抑菌效果,而对安琪酵母菌无抑菌效果。Soluble curdlan oligose with low molecule weight （CRDO） was prepared from curdlan by oxidative degradation of hydrogen peroxide in alkaline homogeneous phase. The effects of NaOH concentration, dosages of H2O2,temperature,and time on curdlan degradation were studied. The optimal hydrolysis conditions were determined as follows： NaOH concentration 2 mol/L, H2O2 dosage 1.5% （V/V）, temperature 40 ℃ and reaction time 48 h. The degradative production was examined by paper chromatography and infrared spectrum analysis, which demonstrated that the chemical structure of the hydrolysates had no change. .In addition, its antitumor activity antibacterial activity and effects on growth performance of Penaeus japonicus were studied. The results showed that it could inhibit the growth of S-180 sarcoma. When CRDO dosage was 50 mg/kg of a rat, the antitumor acitivity was 48.73%. Meanwhile, it could improve the survival rate of Penaeus japonicas, and make the PO activity up to the highest of 2.96 U/mg when CRDO dosage was 400 mg/kg of feed. The antimicrobial tests indicated that the water-soluble CRDO had certain antibacterial effect on staphylococcus aureus and had weak antimicrobial effect on E. coli, but no inhibition on angel yeast.

关 键 词：双氧水 氧化降解 可得然胶 抗肿瘤作用 免疫作用 抑菌作用 

分 类 号：TS201.2[轻工技术与工程—食品科学]