启动子P_(43)表达关键酶基因对subtilosin A合成的调控作用  

Promoter P_(43) regulatory effect for subtilosin A synthesis by expressing key enzyme gene

作　　者：孙会刚[1,2] 张充[1] 吕凤霞[1] 别小妹[1] 陆兆新[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]徐州工程学院食品(生物)工程学院,江苏徐州221000

出　　处：《食品工业科技》2013年第23期164-167,171,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(31271936);河北省自然科学基金资助项目(C2007000191);徐州市科技计划项目(XF11C056);徐州工程学院科研项目(SPY2010209)

摘　　要：为提高枯草芽孢杆菌168(Bacillus subtilis 168)细菌素subtilosin A的产量使其能够应用到食品防腐、医药卫生等行业。采用重叠延伸PCR将强启动子P43分别和subtilosin A合成酶基因簇中关键酶基因sbo-albA、albB-albC连接到一起,构建了高表达载体。采用改进的电击法将重组载体转化到Bacillus subtilis 168中,通过高效液相色谱(HPLC)检测,确定subtilosin A产量得到了提高,进一步通过抑菌实验证明其抑菌活性也获得了提高。该方法为提高subtilosin A产量,进而应用到生产实践中提供了理论参考。For improving Bacillus subtilis 168 bacteriocin subtilosin A production and applying to food preservation, medical science and so on,P43was linked to key enzyme gene sbo-albA,albB-albC of subtilosin A operon using SOE-PCR：High expressing vector was constructed.This recombinant vector was transformed into Bacillus subtilis 168 by high osmolarity electroporation method.HPLC assay showed subtilosin A production was improved.Inhibited method also showed the improving.This method will provide theory reference of improving subtilosin A production and applying in productive practice.

关 键 词：启动子 subtilosin A 关键酶 调控 

分 类 号：TS202.3[轻工技术与工程—食品科学]