大孔树脂纯化酒花多酚及其组成分析  

Macroporous Resin Adsorption for Purification of Hop Polyphenols from and Compositional Analysis

作　　者：王旭苹[1] 杨磊[2] 杨小兰[1] 罗正明[3] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]南开大学生命科学学院,天津300071 [3]忻州师范学院地理旅游管理系,山西忻州034000

出　　处：《食品科学》2013年第22期15-20,共6页Food Science

基　　金：国家自然科学基金面上项目(31171748);山西省科技攻关项目(20090321097)

摘　　要：采用大孔树脂从酿造废酒花中分离纯化酒花多酚,并采用高效液相色谱(HPLC)法分析鉴定了酒花多酚的单酚成分。结果表明:SP850树脂的吸附量为79.8mg/g,解吸率为78.4%,并且达到吸附平衡与解吸平衡的时间较短,较适合酒花多酚的纯化。最佳工艺参数为上样液多酚质量浓度7.3mg/mL、上样量6BV、上样流速3BV/h、洗脱剂为60%乙醇溶液、洗脱流速2BV/h、洗脱剂用量4BV、水洗用量5BV。在此条件下,酒花多酚的纯度由13.2%提高到56.6%。分析表明,酒花多酚纯化物中55.2%的多酚物质是原花青素。HPLC鉴定出酒花多酚中的10种单酚成分,分别为没食子酸、儿茶素、绿原酸、表儿茶素、芦丁、金丝桃苷、紫云英苷、白藜芦醇、异槲皮苷和黄腐酚。Hop polyphenols were extracted from spent hops, purified by macroporous resin adsorption and subjected to compositional analysis by HPLC. The results showed that the adsorption and desorption capacities of hop polyphenols on SP850 resin were 79.8 mg/g and 78.4%, respectively, and the adsorption and desorption processes were equilibrated in a short time, indicating the suitability of this resin for purifying the crude phenols extracted from hops. The optimal purifica- tion conditions were loading of 6 BV of 7.3 mg/mL sample solution at a flow rate of 3 BV/h followed by washing with 5 BV of deionized water and elution with 4 BV of 60% ethanol at a flow rate of 2 BV/h. Under the optimized conditions, the purity of polyphenols in hop extracts was increased from 13.2% to 56.6%. The compositional analysis indicated that 55.2% of the purified polyphenols were proanthocyanidins and 10 individual phenols were identified by HPLC, which were gallic acid, catechin, chlorogenic acid, epicatechin, rutin, hyperoside, astragalin, resveratrol, isoquercitrin and xanthohumol.

关 键 词：酒花多酚 大孔树脂 分离纯化 高效液相色谱 

分 类 号：TS201.1[轻工技术与工程—食品科学]