苹果渣水溶性膳食纤维提取及其对自由基的清除作用  

Extraction of Soluble Dietary Fiber from Apple Pomace and Its Scavenging Capacity against Free Radicals

作　　者：赵明慧[1] 吕春茂[1] 孟宪军[1] 韩路[1] 郑鹏[1] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866

出　　处：《食品科学》2013年第22期75-80,共6页Food Science

基　　金：沈阳市大型仪器共享项目(4130199-1102-01083909001)

摘　　要：为了提高寒富苹果渣中水溶性膳食纤维得率,用α-淀粉酶、木瓜蛋白酶对原料进行预处理,再采用纤维素酶法提取水溶性膳食纤维。在单因素试验基础上,采用响应曲面法Box-Behnken试验设计,对酶解果渣工艺中的纤维素酶添加量、酶解pH值、酶解温度和酶解时间进行优化;研究苹果渣水溶性膳食纤维对DPPH自由基、超氧阴离子自由基(O-2·)和羟自由基(·OH)3种自由基的清除作用。结果表明:纤维素酶法提取的最佳工艺参数为酶添加量1.06%、酶解pH4.9、酶解温度48℃、酶解时间228min,在此条件下苹果渣水溶性膳食纤维得率为(18.21±0.21)%;该水溶性膳食纤维对DPPH自由基、O-2·和·OH均具有一定的清除能力。The extraction of soluble dietary fiber （SDF） from Hanfu apple pomace by hydrolysis with cellulose after pretreatment with α-amylase and papain was optimized by response surface methodology using a Box-Behnken design on the basis of one- factor-at-a-time experiments. Four variables influencing the extraction process, such as enzyme dosage, initial pH, temperature and time were investigated. At the same time, the scavenging effects of apple pomace SDF against DPPH, superoxide anion and hydroxyl free radicals were evaluated. The results indicated that the optimal extraction conditions were 1.06%, 4.9, 48 ℃ and 228 min for enzyme dosage, initial pH, temperature and time, respectively. The yield of SDF under the optimized conditions was （18.21 ±0.21）%. The SDF obtained was able to scavenge all the three free radicals.

关 键 词：寒富苹果渣 水溶性膳食纤维 提取 自由基 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]