常压室温等离子体快速诱变酒精酵母及其突变株的特性研究  

Mutation of alcohol-producing yeast using atmospheric room temperature plasma and the mutant strain characteristic

作　　者：王方方[1] 孙沛勇[1] 银会娟 尹迪[1] 

机构地区：[1]河南天冠企业集团有限公司,河南南阳473000

出　　处：《中国酿造》2013年第10期117-119,共3页China Brewing

摘　　要：常压室温等离子诱变技术可以快速有效的诱变微生物。利用ARTP技术对酵母菌进行诱变,得出其致死时间为90s;通过筛分实验对不同突变标准下的正负突变率进行了统计,得到5株较优良突变株;对其进行多次筛分实验,发酵结果比较一致;遗传稳定性试验结果表明,突变株具有较好的遗传稳定性;通过发酵期间的突变株的失重多于出发菌株的情况验证筛分结果:突变株优于出发菌株。Atmospheric room temperature plasma technique is a quick and effective technique of mutation. The death time of alcohol-producing yeast under atmospheric room temperature plasma was 90s; Five excellent mutant strains were found out by screening experiment and counted the positive or negative mutation rate under different mutation standards. Screening experiments were carded out many times and the fermentation results were quite unanimous. Genetic stability experiments were also carried out and mutant strains were proved to exhibit good genetic stability; the weight loss of mutant strains was more than the original strain, which could also prove the fermentation results.

关 键 词：酒精酵母 诱变 常压室温等离子体 

分 类 号：TE667[石油与天然气工程—油气加工工程]